Polyester microchannel chip for electrophoresis – incorporation of a blue LED as light source
Polyester microchannel chip for electrophoresis – incorporation of a blue LED as light source
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用于电泳的聚酯微通道芯片 – 采用蓝色 LED 作为光源
DOI：
10.1007/s002160100990
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发表时间：
2001
期刊：
影响因子：
--
通讯作者：
T. Hobo
中科院分区：
文献类型：
--
作者：
K. Uchiyama;Wei Xu;Jingmiao Qiu;T. Hobo
抽象的。将蓝色发光二极管作为荧光激发光源并入通过原位聚合制备的聚酯微通道芯片中。将二极管的发光面靠近微通道放置避免了对附加光学布置的任何需要。来自样品的荧光通过与LED垂直的并入微芯片中的光纤传输。在5 mM SDS存在下，通过使用微芯片分离FITC标记的氨基酸，并通过LED诱导的荧光检测。
Abstract. A blue-light-emitting diode was incorporated as a fluorescence-excitation light source into a polyester microchannel chip fabricated by in situ polymerization. Placing the light-emitting facet of the diode close to the microchannel obviated any need for an additional optical arrangement. Fluorescence from the sample was transmitted by an optical fiber incorporated into the microchip perpendicular to the LED. FITC labeled amino acids were separated in the presence of 5 mM SDS by using the microchip and were detected by LED-induced fluorescence.