PATTERNS OF PROTO-ONCOGENE EXPRESSION IN HUMAN GLIOMA CELL-LINES
PATTERNS OF PROTO-ONCOGENE EXPRESSION IN HUMAN GLIOMA CELL-LINES
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DOI：
10.1002/jnr.490240114
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发表时间：
1989-09-01
影响因子：
4.2
通讯作者：
ISRAEL, MA
中科院分区：
文献类型：
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作者：
LAROCCA, RV;ROSENBLUM, M;ISRAEL, MA
先前报道的研究表明，特定肿瘤类型内原癌基因表达模式的变化可能表明这些肿瘤的生物学和临床行为存在潜在差异。为了更灵敏地表征中枢神经系统恶性肿瘤，我们使用 Northern blot 杂交分析来确定从恶性神经胶质瘤患者活检标本建立的 20 个细胞系中 7 个原癌基因的表达模式。以下原癌基因在来自所检查的大多数神经胶质瘤细胞系的 30 μg 总 RNA 中以可检测水平表达：c-myc (20/20)、c-mil/raf-1 (18/18)、neu (19/20)、c-erbB (19/20) 和 c-myb (17/20)。相反，只有一半的细胞系表达可检测的 c-sis (10/20)。在所有测试的细胞系中均未检测到 N-myc (0/20) mRNA。对这 20 个细胞系的形态学分析揭示了三种不同的生长模式：双极、上皮和多形性神经胶质。 可检测水平的 c-sis mRNA 通常出现在上皮或多形性神经胶质形态中。多形性神经胶质亚组的特征还在于神经胶质原纤维酸性蛋白的表达。
Previously reported studies have suggested that variations in the pattern of proto-oncogene expression within a specific tumor type may denote an underlying difference in the biology and clinical behavior of those tumors. To more sensitively characterize malignant tumors of the central nervous system, we have used Northern blot hybridization analysis to determine the patterns of expression of seven proto-oncogenes in 20 cell lines established from biopsy specimens of patients with malignant glioma. The following proto-oncogenes are expressed at detectable levels in 30 .mu.g of total RNA from most glioma cells lines examined: c-myc (20/20), c-mil/raf-1 (18/18), neu (19/20), c-erbB (19/20), and c-myb (17/20). In contrast, only half of the cell lines expressed detectable c-sis (10/20). In none of the cell lines tested was N-myc (0/20) mRNA detected. Morphologic analysis of these 20 cell lines revealed three different growth patterns: bipolar, epithelial, and pleomorphic-glial. Detectable levels of c-sis mRNA typically occurred with either an epithelial or pleomorphic-glial morphology. The pleomorphic-glial subgroup was also characterized by the expression of glial fibrillary acidic protein.