Stable DNA triple helix formation using oligonucleotides containing 2′-aminoethoxy,5-propargylamino-U
Stable DNA triple helix formation using oligonucleotides containing 2′-aminoethoxy,5-propargylamino-U
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DOI：
10.1021/bi020164n
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发表时间：
2002-06-11
期刊：
影响因子：
2.9
通讯作者：
Fox, KR
中科院分区：
文献类型：
--
作者：
Sollogoub, M;Darby, RAJ;Fox, KR
We have prepared oligonucleotides containing the novel base analogue 2'-aminoethoxy,5-proparaylamino-U in place of thymidine and examined their ability to form intermolecular and intramolecular triple helices by DNase I footprinting and thermal melting studies. The results were compared with those for oligonucleotides containing 5-propargylamino-dU and 2'-aminoethoxy-T. We find that the bis-substituted derivative produces a large increase in triplex stability, much greater than that produced by either of the monosubstituted analogues, which are roughly equipotent with each other. Intermolecular triplexes with 9-mer oligonucleotides containing three or four base modifications generate footprints at submicromolar concentrations even at pH 7.5, in contrast to the unmodified oligonucleotide, which failed to produce a footprint at pH 5.0, even at 30 muM. UV- and fluorescence melting studies with intramolecular triplexes confirmed that the bis-modified base produces a much greater increase in T-m than either modification alone.
