Biochemical analyses of ppGpp effect on adenylosuccinate synthetases, key enzymes in purine biosynthesis in rice
Biochemical analyses of ppGpp effect on adenylosuccinate synthetases, key enzymes in purine biosynthesis in rice
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DOI：
10.1080/09168451.2014.910103
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发表时间：
2014-06
期刊：
影响因子：
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通讯作者：
Yuhta Nomura;A. Nozawa;Y. Tozawa
中科院分区：
文献类型：
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作者：
Yuhta Nomura;A. Nozawa;Y. Tozawa
ppGpp信号系统在植物叶绿体中发挥作用。在细菌中，ppGpp对腺苷酸琥珀酸合成酶（AdSS）有负面影响。我们的生化分析还显示水稻AdSS同源物对ppGpp明显敏感。然而，进一步的研究表明，这种现象被酶的高底物亲和力所抵消，导致ppGpp对腺苷酸琥珀酸合成的影响有限。
The ppGpp-signaling system functions in plant chloroplasts. In bacteria, a negative effect of ppGpp on adenylosuccinate synthetase (AdSS) has been suggested. Our biochemical analysis also revealed rice AdSS homologs are apparently sensitive to ppGpp. However, further investigation clarified that this phenomenon is cancelled by the high substrate affinity to the enzymes, leading to a limited effect of ppGpp on adenylosuccinate synthesis.