Acid-sensing ion channel-2 is not necessary for sour taste in mice
Acid-sensing ion channel-2 is not necessary for sour taste in mice
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DOI：
10.1523/jneurosci.0653-04.2004
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发表时间：
2004-04-21
影响因子：
5.3
通讯作者：
Chaudhari, N
中科院分区：
文献类型：
--
作者：
Richter, TA;Dvoryanchikov, GA;Chaudhari, N
酸敏感阳离子通道酸敏感离子通道2（ASIC 2）的生理特性和在大鼠味蕾细胞中的表达被广泛认为是哺乳动物中酸（酸）味的受体。使用逆转录-PCR，我们检测ASIC 1和ASIC 3的表达，但没有ASIC 4，在小鼠和大鼠的味蕾和非感觉舌上皮。令人惊讶的是，我们在小鼠味蕾中没有检测到ASIC 2的mRNA，尽管我们很容易观察到它在大鼠味蕾中的表达。此外，在Ca 2+成像实验中，ASIC 2敲除小鼠对酸味刺激表现出正常的生理反应（细胞内Ca 2+浓度增加）。我们的研究结果表明，ASIC 2是不需要在小鼠的酸味，如果一个通用的哺乳动物酸味转导机制存在，它可能使用其他酸敏感受体或离子通道。
The acid-sensitive cation channel acid-sensing ion channel-2 (ASIC2) is widely believed to be a receptor for acid (sour) taste in mammals on the basis of its physiological properties and expression in rat taste bud cells. Using reverse transcriptase-PCR, we detected expression of ASIC1 and ASIC3, but not ASIC4, in mouse and rat taste buds and nonsensory lingual epithelium. Surprisingly, we did not detect mRNA for ASIC2 in mouse taste buds, although we readily observed its expression in rat taste buds. Furthermore, in Ca2+ imaging experiments, ASIC2 knock-out mice exhibited normal physiological responses (increases in intracellular Ca2+ concentrations) to acid taste stimuli. Our results indicate that ASIC2 is not required for acid taste in mice, and that if a universal mammalian acid taste transduction mechanism exists, it likely uses other acid-sensitive receptors or ion channels.