Identification and characterization of autoantibody-producing B220low B (B-1) cells appearing in malarial infection
Identification and characterization of autoantibody-producing B220low B (B-1) cells appearing in malarial infection
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DOI：
10.1016/j.cellimm.2010.02.015
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发表时间：
2010-01-01
影响因子：
4.3
通讯作者：
Abo, Toru
中科院分区：
文献类型：
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作者：
Kanda, Yasuhiro;Kawamura, Hiroki;Abo, Toru
患疟疾的小鼠表现出独特的免疫反应，包括NK1.1（-）TCR（int）细胞（胸腺外T细胞）的扩增。由于具有自身反应性的TCRint细胞和自身抗体产生性B细胞（B-1细胞）在自身免疫条件下经常同时活化，因此检测了B-1细胞在疟疾感染过程中是否活化。从感染后第14天开始，在肝脏和脾脏中出现B220（低）B-1细胞。B220（低）B细胞的数量在第14天最高，但比例在第28-35天最高。与B220（低）细胞的出现平行，在血清中检测到针对HEp-2细胞和双链DNA的自身抗体。这些B220（低）细胞具有CD 44（高）、CD 23（-）和CD 62 L（-）表型。与此形成鲜明对比的是，常规B220（高）B细胞（B-2细胞）为CD 44（低）、CD 23（+）和CD 62 L（+）。这些结果表明，疟疾免疫应答不是由传统的T和B细胞介导的，而是类似于自身免疫性疾病期间的应答。(C)2010年爱思唯尔公司All rights reserved.
Mice with malaria showed unique immunological responses, including the expansion of NK1.1(-)TCR(int) cells (extrathymic T cells). Since TCRint cells with autoreactivity and autoantibody-producing B cells (B-1 cells) are often simultaneously activated under autoimmune conditions, it was examined whether B-1 cells were activated in the course of malarial infection. From days 14 after infection, B220(low) B-1 cells appeared in the liver and spleen. The number of B220(low) B cells was highest at day 14, but the ratio was highest at days 28-35. In parallel with the appearance of B220(low) cells, autoantibodies against HEp-2 cells and double-stranded DNA were detected in sera. These B220(low) cells had phenotypes of CD44(high), CD23(-) and CD62L(-). In sharp contrast, conventional B220(high) B cells (B-2 cells) were CD44(low), CD23(+) and CD62L(+). These results suggested that malaria immune responses were not mediated by conventional T and B cells but resembled the responses during autoimmune diseases. (C) 2010 Elsevier Inc. All rights reserved.