Validation of the lactose-[13C]ureide breath test for determination of orocecal transit time by scintigraphy.
Validation of the lactose-[13C]ureide breath test for determination of orocecal transit time by scintigraphy.
复制标题
验证乳糖-[13C]脲呼气试验通过闪烁扫描确定口盲肠通过时间。
DOI：
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发表时间：
1999
影响因子：
9.3
通讯作者：
P. Rutgeerts
中科院分区：
文献类型：
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作者：
B. Geypens;R. Bennink;M. Peeters;P. Evenepoel;L. Mortelmans;B. Maes;Y. Ghoos;P. Rutgeerts
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口服13C标记底物乳糖缩脲(LU)进行呼气试验，以测量口盲部通过时间(OCTT)，并与99mTc-核素扫描进行对照。虽然LU不被人体小肠吸收，但结肠菌很容易代谢LU，产生13C标记的二氧化碳。13CO2出现在呼气中的时间与OCTT相对应。
方法
22名健康志愿者摄入了一份标有99mTc和13C-LU的食物。以10分钟或15分钟的时间间隔进行8h以上的核素扫描。OCTT的闪烁成像被定义为至少10%的标记已经进入结肠的时间。每隔10~15min采集一次呼气样本，持续10h，采用同位素比值质谱仪进行测定。OCTT被定义为首次显著高于基线的时间。结果用相关性和Altman-Bland统计方法进行比较。
结果
OCTT与呼气试验结果有良好的相关性(r=0.94)。Altman-Bland的统计数据显示，核素扫描和呼气测试的结果非常一致。男性和女性受试者之间没有显著差异。
结论
使用13C-LU的呼气试验是测量OCTT的一种有效的替代闪烁成像技术。
UNLABELLED
The breath test using oral administration of a 13C-labeled substrate, lactose-ureide (LU), to measure orocecal transit time (OCTT) was validated against 99mTc-scintigraphy. Although LU is not absorbed in the human small intestine, colonic bacteria readily metabolize LU, producing 13C-labeled CO2. The time at which 13CO2 appears in breath corresponds to the OCTT.
METHODS
Twenty-two healthy volunteers ingested a meal labeled with 99mTc and 13C-LU. Scintigraphy was performed over 8 h at time intervals of 10 or 15 min. OCTT with scintigraphy was defined as the time at which at least 10% of the label had entered the colon. Breath samples were obtained every 10-15 min for 10 h and measured by isotope ratio mass spectrometry. OCTT was defined as the time of first significant increase above baseline. The results were compared using correlation and Altman-Bland statistics.
RESULTS
OCTT results from scintigraphy (mean OCTT = 283+/-53 min) and breath test (mean OCTT = 292+/-58 min) correlated well (r = 0.94). Altman-Bland statistics showed close agreement between scintigraphy and breath test. No significant difference between male and female subjects was observed.
CONCLUSION
The breath test using 13C-LU is a valid alternative to scintigraphy techniques for measuring OCTT.