Analyzing stochastic events in multi-channel patch clamp data.
Analyzing stochastic events in multi-channel patch clamp data.
复制标题
分析多通道膜片钳数据中的随机事件。
DOI：
10.1007/bf00363953
复制
发表时间：
1988
影响因子：
1.9
通讯作者：
Chay,SC
中科院分区：
文献类型：
--
作者：
Chay,TR;Kang,HS;Chay,SC
为了了解生物膜离子通道的门控特性，发展了一种有效的方法来估算通道的开-关动力学常数。该方法适用于多个离子通道同时工作的单通道膜片钳记录。与Horn和Lange提出的极大似然法不同，本文的方法是一种连续的方法，并利用了事件时间的概率密度函数的解析表达式。组合分析是必要的，以正确地包括更典型的多通道记录。这比Horn和Lange使用离散化时间序列的方法产生更快的计算结果。代码中依赖于模型的部分非常少，并且很容易修改。为了说明我们的方法的优点，我们使用指数随机数发生器在数字计算机上生成了膜片钳记录的开-关过程。对于多通道补丁，我们已经引入了一些合理的近似，使我们的算法更有效。我们的近似的合理性进行了测试，如开放状态的分数在timet，popen（t），和频率的开口次数每次运行等措施。实现该算法的计算机代码的副本可以从作者处获得。
In an effort to understand the gating properties of ionic channels in biological membranes, an efficient method was developed to estimate the kinetic constants from opening-closing events of the channels. Our method is suitable to single channel patch-clamp recordings that contain several ionic channels functioning simultaneously. It is different from the maximum likelihood method previous developed by Horn and Lange, in that our method is a continuum approach and makes uses of analytic expressions of the probability density functions of the event times. Combinatorial analysis was necessary to correctly include more typical multi-channel recordings. This yields computationally quicker results than the method of Horn and Lange, which uses a discretized time series. Model-dependent portions of the code are minimal and easily modified. To illustrate the goodness of our method, we have generated the open-close processes of the patch-clamp records on a digital computer using the exponential random number generators. For multi-channel patches, we have introduced a few plausible approximations to make our algorithm more efficient. The soundness of the our approximations were tested with such measures as the fraction of the open state at timet,popen(t), and frequencies of the number of openings per run. A copy of the computer code implementing this algorithm can be obtained from the authors.