ENTRY OF MALARIA PARASITES INTO RESEALED GHOSTS OF HUMAN AND SIMIAN ERYTHROCYTES
ENTRY OF MALARIA PARASITES INTO RESEALED GHOSTS OF HUMAN AND SIMIAN ERYTHROCYTES
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DOI：
10.1111/j.1365-2141.1981.tb07201.x
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发表时间：
1981-01-01
影响因子：
6.5
通讯作者：
GRATZER, WB
中科院分区：
文献类型：
--
作者：
DLUZEWSKI, AR;RANGACHARI, K;GRATZER, WB
传统上通过低渗裂解和洗涤制备的白幽灵能够抵抗疟原虫的侵袭，但是在高血细胞比容下裂解并重新密封而不将膜与细胞质分离的细胞能够高效地侵入（不低于完整细胞频率的1/3）。分别暴露于镰状疟原虫和诺氏疟原虫的人类和猴子细胞也获得了类似的结果。使用单个细胞的显微分光光度测定法来确定裂解、重新密封的细胞对侵袭的敏感性与其血红蛋白含量无关。
White ghosts, conventionally prepared by hypotonic lysis and washing are resistant to invasion by malaria parasites, but cells lysed at high hematocrit and resealed without separating the membranes from the cytosol are invaded with high efficiency (not less than 1/3 the frequency of intact cells). Similar results were obtained with human and monkey cells, exposed, respectively, to Plasmodium flaciparum and P. knowlesi. Microspectrophotometry of individual cells was used to ascertain that the susceptibility to invasion of lysed, resealed cells was not related to its Hb content.