Soil microbial community changes as a result of long-term exposure to a natural CO2 vent
Soil microbial community changes as a result of long-term exposure to a natural CO2 vent
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DOI：
10.1016/j.gca.2010.02.006
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发表时间：
2010-05-01
影响因子：
5
通讯作者：
Krueger, M.
中科院分区：
文献类型：
--
作者：
Oppermann, B. I.;Michaelis, W.;Krueger, M.
二氧化碳的捕获和地质储存可用于减少人为温室气体排放。为了评估深层水库潜在的二氧化碳泄漏对近地表陆地环境中微生物丰度和功能多样性的环境影响，以天然二氧化碳排放口(土壤气体中90%的二氧化碳)为模拟对象进行了研究。使用脂类生物标志物结合化合物特有的稳定碳同位素分析、微生物活性测定和定量聚合酶链式反应(Q-PCR)对微生物群落进行了调查。通过这套互补的方法，在富含二氧化碳的喷口和正常二氧化碳浓度的参照点之间检测到了显著的差异。二氧化碳喷口内植物和微生物脂肪的增量C-13值表明，大量的地热二氧化碳被并入微生物、植物和土壤碳库。此外，古细菌和细菌的数量在参照点最高，而在二氧化碳排放口显著较低。脂类生物标记物分析、Q-PCR和微生物活性的测定表明，利用CO2的古细菌、地质杆菌科和硫酸盐还原细菌(SRB)主要存在于二氧化碳排放口，在参考部位仅有少量存在。稳定碳同位素分析表明，产甲烷古生菌和SRB利用通风口产生的二氧化碳进行同化生物合成。我们的结果表明，由于土壤环境长期暴露在高二氧化碳浓度下，微生物群落向厌氧和嗜酸微生物转变。(C)2010爱思唯尔有限公司。所有帽子保留。
The capture and geological storage of CO2 can be used to reduce anthropogenic greenhouse gas emissions. To assess the environmental impact of potential CO2 leakage from deep storage reservoirs on the abundance and functional diversity of microorganisms in near-surface terrestrial environments, a natural CO2 vent (>90% CO2 in the soil gas) was studied as an analogue. The microbial communities were investigated using lipid biomarkers combined with compound-specific stable carbon isotope analyses, the determination of microbial activities, and the use of quantitative polymerase chain reactions (Q-PCR). With this complementary set of methods, significant differences between the CO2-rich vent and a reference site with a normal CO2 concentration were detected. The delta C-13 values of the plant and microbial lipids within the CO2 vent demonstrate that substantial amounts of geothermal CO2 were incorporated into the microbial, plant, and soil carbon pools. Moreover, the numbers of Archaea and Bacteria were highest at the reference site and substantially lower at the CO2 vent. Lipid biomarker analyses, Q-PCR, and the determination of microbial activities showed the presence of CO2-utilising methanogenic Archaea, Geobacteraceae, and sulphate-reducing Bacteria (SRB) mainly at the CO2 vent, only minor quantities were found at the reference site. Stable carbon isotopic analyses revealed that the methanogenic Archaea and SRB utilised the vent-derived CO2 for assimilatory biosynthesis. Our results show a shift in the microbial community towards anaerobic and acidophilic microorganisms as a consequence of the long-term exposure of the soil environment to high CO2 concentrations. (C) 2010 Elsevier Ltd. All Hats reserved.