Mechanisms that ensure monogamous mating in Saccharomyces cerevisiae.
Mechanisms that ensure monogamous mating in Saccharomyces cerevisiae.
复制标题
确保酿酒酵母中一夫一妻制交配的机制。
DOI：
10.1091/mbc.e20-12-0757
复制
发表时间：
2021-04-15
影响因子：
3.3
通讯作者：
Lew DJ
中科院分区：
文献类型：
--
作者：
Robertson CG;Clark-Cotton MR;Lew DJ
芽殖酵母酿酒酵母的单倍体细胞利用分泌的信息素进行交流，并交配形成二倍体合子。交配是一夫一妻制的，导致每种交配类型的一个细胞恰好融合。在拥挤的条件下，一夫一妻制的交配，细胞可以接触到不止一个潜在的伴侣，这就提出了如何防止多次交配的问题。在这里，我们确定的突变体能够与多个合作伙伴，揭示机制，确保一夫一妻制交配。在融合之前，细胞发展出朝向潜在伴侣的极性灶。每个细胞内这些极性焦点之间的竞争导致除一个焦点之外的所有焦点的解体，从而有利于单个融合事件。融合促进在每种交配类型中独特表达的亚基之间形成异二聚体复合物。一个复合体关闭了单倍体特异性基因的表达，另一个关闭了对信息素的反应能力。能够形成任一复合体的合子保持一夫一妻制，但缺乏两者的合子可以重新交配。
Haploid cells of the budding yeast Saccharomyces cerevisiae communicate using secreted pheromones and mate to form diploid zygotes. Mating is monogamous, resulting in the fusion of precisely one cell of each mating type. Monogamous mating in crowded conditions, where cells have access to more than one potential partner, raises the question of how multiple-mating outcomes are prevented. Here we identify mutants capable of mating with multiple partners, revealing the mechanisms that ensure monogamous mating. Before fusion, cells develop polarity foci oriented toward potential partners. Competition between these polarity foci within each cell leads to disassembly of all but one focus, thus favoring a single fusion event. Fusion promotes the formation of heterodimeric complexes between subunits that are uniquely expressed in each mating type. One complex shuts off haploid-specific gene expression, and the other shuts off the ability to respond to pheromone. Zygotes able to form either complex remain monogamous, but zygotes lacking both can re-mate.