Apotosis-related proteins in cervical intraepithelial neoplasia and squamous cell carcinoma of the cervix
Apotosis-related proteins in cervical intraepithelial neoplasia and squamous cell carcinoma of the cervix
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DOI：
10.1006/gyno.2002.6655
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发表时间：
2002-07-01
影响因子：
4.7
通讯作者：
Wong, YF
中科院分区：
文献类型：
--
作者：
Cheung, TH;Chung, TKH;Wong, YF
目标。增殖和凋亡之间失去平衡会导致肿瘤形成。正常宫颈上皮在可能发展为癌之前会发生发育异常。本研究的目的是描述凋亡相关蛋白在宫颈肿瘤不同发展阶段的作用。对调节蛋白和效应蛋白均进行了检查。方法。使用免疫组织化学方法评估了 26 例低级别宫颈上皮内瘤变 (CIN-L)、37 例高级别宫颈上皮内瘤变 (CIN-H) 和 43 例宫颈鳞状细胞癌中凋亡相关蛋白（包括 Bcl-2 家族的 5 个成员和 caspase 家族的 2 个成员）的表达。在 10% 或更多的肿瘤细胞中显示出胞质免疫反应性的样本被认为是免疫阳性的。结果。研究了一百六个科目。在这些肿瘤的一部分中，所有七个检查的细胞凋亡调节因子均呈阳性。 CIN-H 中 Caspase 3 的表达显着高于 CIN-L（P = 0.016）。与CIN-H相比，宫颈癌中Bak、Caspase 3和Caspase 6的表达降低（分别为P < 0.01、P = 0.026、P < 0.01）。结论。与 CIN-L 相比，CIN-H 中 Caspase 3 的表达增加，并且这种增加被认为与发育异常细胞的增殖活性增加有关。癌组织中Bax、Caspase 3、Caspase 6表达的减少表明细胞癌变过程中细胞凋亡机制出现缺陷。 (C) 2002 年爱思唯尔科学（美国）。
Objectives. A loss of balance between proliferation and apoptosis leads to tumor formation. Normal cervical epithelium becomes dysplastic before potentially developing into carcinoma. This study was conducted to delineate the role of apoptosis-related proteins in various stages of development in cervical neoplasia. Both regulator and effector proteins were examined.Methods. The expression of apoptosis-related proteins, including five members of the Bcl-2 family and two members of the caspase family, was evaluated in 26 low-grade cervical intraepithelial neoplasias (CIN-L), 37 high-grade cervical intraepithelial neoplasias (CIN-H), and 43 cervical squamous cell carcinomas, using immunohistochemistry. Specimens showing cytosolic immunoreactivity in 10% or more of the neoplastic cells were considered immunopositive.Results. One hundred six subjects were studied. All seven apoptotic regulators examined were positive in a proportion of these neoplasms. The expression of Caspase 3 was significantly higher in CIN-H than in CIN-L (P = 0.016). The expression of Bak, Caspase 3, and Caspase 6 was reduced in cervical carcinoma compared to CIN-H (P < 0.01, P = 0.026, P < 0.01, respectively).Conclusions. There is an increase in expression of Caspase 3 in CIN-H compared to CIN-L and the increase is thought to be related to the increased proliferative activity in dysplastic cells. The reduction of Bax, Caspase 3, and Caspase 6 expression in carcinoma indicates that the apoptotic mechanism has become defective in the process of malignant transformation. (C) 2002 Elsevier Science (USA).