WATER-SOLUBLE ARABINOGALACTAN-PEPTIDE FROM WHEAT ENDOSPERM
WATER-SOLUBLE ARABINOGALACTAN-PEPTIDE FROM WHEAT ENDOSPERM
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DOI：
10.1071/bi9740117
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发表时间：
1974-01-01
期刊：
影响因子：
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通讯作者：
STONE, BA
中科院分区：
文献类型：
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作者：
FINCHER, GB;STONE, BA
用两种不同的方法提取了小麦胚乳的水溶性聚合物组分，并对其化学组成进行了详细的研究。首先用热的80%乙醇处理的小麦粉的水提取物仅含有2%的蛋白质，但是如果省略乙醇处理，则高达20%的提取的聚合部分是低分子量的不可透析的蛋白质材料。氯化铯溶液中的密度梯度超离心表明，这种蛋白质的大部分是免费的，而2%的蛋白质在乙醇处理的flottr的水提取物牢固地结合到多糖。该结合蛋白（肽）的特征在于高水平的羟脯氨酸（存在的氨基酸的16-20%）。
The water-soluble polymeric components of wheat endosperm have been extracted by two different procedures and their chemical composition studied in detail. Water extracts of wheat flour that had first been treated with hot 80% ethanol contained only 2% protein, but if the ethanol treatment was omitted up to 20% of the extracted polymeric fraction was low-molecular-weight non-dialysable protein material. Density-gradient ultracentrifugation in caesium chloride solutions indicated that most of this protein was free, whereas the 2% protein in the water extract of ethanol-treated flottr was firmly bound to a polysaccharide. This bound protein (a peptide) was characterized by high levels of hydroxyproline (16-20% of the amino acids present).