Retrograde Tracing with Fluorescent Microspheres Reveals Bifurcating Projections from Central Retina to Tectum and Thalamus in Chicks
Retrograde Tracing with Fluorescent Microspheres Reveals Bifurcating Projections from Central Retina to Tectum and Thalamus in Chicks
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荧光微球逆行追踪揭示了雏鸡从中央视网膜到顶盖和丘脑的分叉投影
DOI：
10.1111/j.1439-0264.2011.01131.x
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发表时间：
2012-08-01
影响因子：
0.9
通讯作者：
Chen, Y.
中科院分区：
文献类型：
--
作者：
Cao, J.;Naito, J.;Chen, Y.
本研究的目的是利用荧光逆行示踪技术证实鸡视网膜神经节细胞（retinal ganglion cells，RGC）向顶盖和视丘脑的双重投射模式，并通过荧光黄（Lucifer Yellow，LY）胞内注射结合免疫组织化学方法，明确具有双重投射的RGC的形态学特征。32只鸡接受绿色和红色荧光微球的双重注射到他们的丘脑和顶盖在同侧。在视网膜中央部，大部分标记的RGC为tec-RGC（投射到顶盖的RGC），四分之一为tha-RGC（投射到丘脑的RGC），几乎所有的tha-RGC都是双标记的RGC。将LY细胞内注射到双标记的RGC中显示了所有六组RGC，每组中没有特异性群体（J.Comp.Neurol.，2004，469：360）。这些树枝状结构多为单层和双层，在内丛状层较深处水平延伸。
The goal of this study is to demonstrate the dual-projection pattern of retinal ganglion cells (RGCs) projecting to the tectum and visual thalamus in chick using retrograde fluorescent tracers and also to define the morphological properties of these RGCs with dual projections by intracellular injection of Lucifer Yellow (LY) combined with immunohistochemistry. Thirty-two chicks received double injections of green and red fluorescent microspheres into their thalamus and tectum in the same side. In the central retina, most of the labelled RGCs were tec-RGCs (RGCs projecting to the tectum), a quarter was tha-RGCs (RGCs projecting to the thalamus), and almost all of the tha-RGCs were double-labelled RGCs. An intracellular injection of LY into the double-labelled RGCs showed all six groups of RGCs without specific populations in each group (J. Comp. Neurol., 2004, 469: 360). These dendritic patterns were mostly mono- and bistrata, which extended horizontally in the deeper part of the inner plexiform layer.