Distinct Distribution of the Tensin Family in the Mouse Kidney and Small Intestine
Distinct Distribution of the Tensin Family in the Mouse Kidney and Small Intestine
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DOI：
10.1538/expanim.61.525
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发表时间：
2012-10-01
影响因子：
2.4
通讯作者：
Agui, Takashi
中科院分区：
文献类型：
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作者：
Nishino, Tomohiro;Sasaki, Nobuya;Agui, Takashi
张力蛋白家族成员是定位于整合素介导的细胞-基底膜连接处并涉及细胞骨架组织、细胞迁移和增殖的细胞质蛋白。哺乳动物基因组包含四个旁系同源物，Tns 1，Tns 2，Tns 3和Tns 4。小鼠Tns 1和Tns 2基因突变分别导致多囊肾病和肾小球硬化，Tns 3缺失小鼠表现出肠上皮发育受损。然而，关于每个张力的定位知识仍然是零碎的。在这项研究中，张力成员的细胞和亚细胞分布的定义和相互比较。RT-PCR分析表明，Tns 2和Tns 3在离体肾小球中表达较丰富，Tns 1在肾小球以外的区域高表达，但在肾脏中未观察到Tns 4表达。在小肠中检测到所有张力元件。免疫组织化学染色显示，Tns 1主要定位于肾小球系膜和肾小管。相反，Tns 2和Tns 3在足细胞和部分收集系统中高表达。在小肠中，Tns 2和Tns 3在隐窝和绒毛上皮细胞中高表达。此外，我们发现Tns 3与TJ蛋白ZO-1共定位于肾小管。这些结果表明Tns 1，Tns 2和Tns 3的细胞表达存在明显差异，并表明它们可能能够在肾脏和小肠中相互独立地发挥作用。
Tensin family members are cytoplasmic proteins that are localized to the integrin-mediated cell-basement membrane junctions and implicated in cytoskeletal organization, cell migration, and proliferation. The mammalian genome contains four paralogs, Tns1, Tns2, Tns3, and Tns4. Murine mutations in the Tns1 and Tns2 genes cause polycystic kidney disease and glomerular sclerosis, respectively, and Tns3-null mice exhibit an impaired intestinal epithelial development. However, the knowledge concerning the localization of each tensin is still fragmentary. In this study, the cellular and subcellular distributions of tensin members were defined and compared with each other. RT-PCR analysis indicated that Tns2 and Tns3 were more abundant in isolated glomeruli and that Tns1 was highly expressed in areas other than the glomeruli, but no Tns4 expression was observed in the kidney. All tensin members were detected in the small intestine. Immunohistochemical staining revealed that Tns1 was predominantly localized to the mesangium of glomeruli and renal tubules. In contrast, Tns2 and Tns3 were highly expressed in the podocytes and the partial collecting system. In the small intestine, Tns2 and Tns3 were highly expressed in crypt and villous epithelial cells. Furthermore, we found that Tns3 was colocalized with TJ protein ZO-1 in renal tubules. These results indicate distinct differences in the cellular expression of Tns1, Tns2, and Tns3, and suggest that they may be able to function independently of each other in the kidney and the small intestine.