Iron-chelating ability and antioxidant properties of phycocyanin isolated from a protean extract of Spirulina platensis
Iron-chelating ability and antioxidant properties of phycocyanin isolated from a protean extract of Spirulina platensis
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DOI：
10.1016/j.foodchem.2008.02.021
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发表时间：
2008-09-15
期刊：
影响因子：
8.8
通讯作者：
Villar, Angel M.
中科院分区：
文献类型：
--
作者：
Bermejo, Paloma;Pinero, Enrique;Villar, Angel M.
研究了不同活性氧自由基(超氧阴离子自由基、羟基自由基、过氧化氢自由基、次氯酸和过氧化氢自由基)的体外清除活性，以及钝顶螺旋藻蛋白质提取物和从该微藻中分离的胆蛋白藻蓝蛋白对脂质过氧化和铁络合能力的影响。钝顶螺旋藻蛋白提取物对羟基自由基的产生(加入EDTA的IC50=537 mU g/ml，不加EDTA的IC50=1500 mU g/ml)、过氧化氢自由基的产生(IC50=230 mU g/ml)、脂质过氧化反应(酶体系的IC50=2320 mU g/ml，非酶体系的IC50=2180 mU g/ml)均有抑制作用。此外，藻蓝蛋白还能抑制羟基和过氧化自由基及脂质过氧化过程。铁离子降低了钝顶螺旋藻蛋白提取物和藻蓝蛋白的最大荧光发射光谱，是金属络合活性的指示剂。钝顶螺旋藻和藻蓝蛋白的抗氧化作用可能来源于清除自由基和金属络合作用。我们的结果表明，钝顶螺旋藻可以作为一种膳食补充剂来预防一些涉及自由基的疾病。(C)2008爱思唯尔有限公司。保留所有权利。
The in vitro scavenger activities of different reactive oxygen species (superoxide radical, hydroxyl radical, hydrogen peroxide, hypochlorous acid and peroxyl radical), the effects on lipid peroxidation and the iron-chelating ability of a Spirulina platensis protean extract and the biliprotein, phycocyanin, isolated from this microalga were studied. S. platensis protean extract inhibited the generation of hydroxyl radical (IC50 = 537 mu g/ml for the system with EDTA and 1500 mu g/ml without EDTA), the production of peroxyl radical (IC50 = 230 mu g/ml), and the lipid peroxidation process IC50 = 2320 mu g/ml for the enzymatic system and 2180 mu g/ml for the non-enzymatic system). Besides, phycocyanin inhibited hydroxyl and peroxyl radicals and the lipid peroxidation process. The iron ions decreased the maximum fluorescence emission spectra of S. platensis protean extract and phycocyanin and it was an indicator of the metal-chelating activity. The antioxidant properties of S. platensis and phycocyanin may arise from both radical-scavenging and metal chelation. Our results suggest that S. platensis could be used as a dietary supplement to prevent some diseases where free radicals are involved. (c) 2008 Elsevier Ltd. All rights reserved.