Synthesis and characterization of organosilica nanoparticles prepared from 3-mercaptopropyltrimethoxysilane as the single silica source
Synthesis and characterization of organosilica nanoparticles prepared from 3-mercaptopropyltrimethoxysilane as the single silica source
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DOI：
10.1021/jp075798o
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发表时间：
2007-12-27
影响因子：
3.7
通讯作者：
Ishimura, Kazunori
中科院分区：
文献类型：
--
作者：
Nakamura, Michihiro;Ishimura, Kazunori
以3-巯丙基三甲氧基硅烷为硅源，采用Stober法合成了新型有机硅纳米粒子。MPS纳米颗粒在溶液中分散良好，并且具有独特的表面性质，例如表面上的硫醇残基和与由四乙氧基硅烷制成的纳米颗粒相比降低的zeta电位。通过在二氧化硅网络上沉积荧光染料的方法合成具有荧光染料的MPS纳米颗粒。所得具有荧光的MPS NP是明亮的，在溶液中不聚集，并且是光稳定的。荧光MPS NP的荧光强度和光稳定性足以使用流式细胞术和荧光显微术作为单个荧光颗粒进行检测。通过吸附和马来酰亚胺偶联容易地制备蛋白质修饰的MPS NP。在本文中，我们展示了MPS纳米粒子的有用性和它们的表面性质，并讨论了它们在生物应用中的优势。
Novel organosilica nanoparticles made of 3-mercaptopropyltrimethoxysilane as the single silica source (MPS NPs) have been synthesized successfully by using a Stober method. The MPS NPs were well dispersed in solution and have unique surface properties such as thiol residues on the surface and reduced zeta potential compared with the nanoparticles made of tetraethoxysilane. The MPS NPs with fluorescent dye were synthesized by a method that deposited fluorescent dye on the silica network. The resulting MPS NPs with fluorescence were bright, nonaggregated in solution, and photostable. The fluorescence intensity and photostability of fluorescent MPS NPs were sufficient for detection as a single fluorescent particle using flow cytometry and fluorescence microscopy. Protein-modified MPS NPs were prepared easily by absorption and by maleimide coupling. In this paper, we demonstrate the usefulness of MPS NPs and their surface properties and discuss their advantages for biological applications.