Changes in the uterine metabolome of the cow during the first 7 days after estrus.
Changes in the uterine metabolome of the cow during the first 7 days after estrus.
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母牛发情后前 7 天内子宫代谢组的变化。
DOI：
10.1002/mrd.23082
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发表时间：
2019
影响因子：
2.5
通讯作者：
Hansen,PeterJ
中科院分区：
文献类型：
--
作者：
Tríbulo,Paula;Balzano-Nogueira,Leandro;Conesa,Ana;Siqueira,LuizG;Hansen,PeterJ
排卵后头7天的子宫微环境可容纳并促进精子进入输卵管，是植入前胚胎发育所需营养的唯一来源。对宫液成分的了解在很大程度上是不完整的。使用非靶向质谱仪，我们描述了发情周期的前7天的子宫代谢物。于排卵前第0天(N= 4)、第3天(N= 4)、第5天(N= 3)和第7天(N= 4)采集牛子宫，用杜贝克磷酸盐缓冲液冲洗。共检测到1,993个分子特征，其中184个峰可能被鉴定为147种独特的代谢物，包括氨基酸、苯甲酸、脂分子、碳水化合物、嘌呤、嘧啶、维生素和其他中间和次生代谢物。结果显示，当奶牛从排卵过渡到发情周期的第7天时，子宫代谢物发生了变化。大多数代谢物随天变化的强度在排卵的第5天或第7天达到最大。此外，在子宫液中发现的几种代谢物具有信号传递功能，其中一些已被证明影响植入前胚胎的发育。综上所述，牛子宫的代谢组在发情周期的早期阶段发生变化，并可能参与着床前胚胎发育的调节。这里报告的数据将作为未来研究的基础，旨在评估植入前胚胎发育的母体调节和胚胎培养的最佳条件。
The uterine microenvironment during the first 7 days after ovulation accommodates and facilitates sperm transit to the oviduct and constitutes the sole source of nutrients required for the development of preimplantation embryos. Knowledge of the composition of uterine fluid is largely incomplete. Using untargeted mass spectrometry, we characterized the uterine metabolome during the first 7 days of the estrous cycle. Bovine uteri were collected on Days 0 (N= 4), 3 (N= 4), 5 (N= 3), and 7 (N= 4) relative to ovulation and flushed with Dulbecco’s phosphate‐buffered saline. A total of 1,993 molecular features were detected of which 184 peaks with putative identification represent 147 unique metabolites, including amino acids, benzoic acids, lipid molecules, carbohydrates, purines, pyrimidines, vitamins, and other intermediate and secondary metabolites. Results revealed changes in the uterine metabolome as the cow transitions from ovulation to Day 7 of the estrous cycle. The majority of metabolites that changed with day reached maximum intensity on either Day 5 or 7 relative to ovulation. Moreover, several metabolites found in the uterine fluid have signaling capabilities and some have been shown to affect preimplantation embryonic development. In conclusion, the metabolome of the bovine uterus changes during early stages of the estrous cycle and is likely to participate in the regulation of preimplantation embryonic development. Data reported here will serve as the basis for future studies aiming to evaluate maternal regulation of preimplantation embryonic development and optimal conditions for the culture of embryos.