Dielectrophoretic force microscopy of aqueous interfaces
Dielectrophoretic force microscopy of aqueous interfaces
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DOI：
10.1021/la0484366
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发表时间：
2005-02-15
期刊：
影响因子：
3.9
通讯作者：
Simpson, GJ
中科院分区：
文献类型：
--
作者：
Lynch, BP;Hilton, AM;Simpson, GJ
一种新的扫描探针显微镜技术使得介电泳力成像具有纳米级的空间分辨率。介电电泳（DEP）传统上描述极化粒子在非均匀交流电场中的迁移率。将DEP与原子力显微镜相结合，可以实现与局部电极化率相关的图像对比度的非接触成像。通过调整交流频率，介质光谱可以在高空间分辨率的固体/液体界面上进行。在细胞的研究中，频率依赖的介电泳力对生物相关的电学特性很敏感，包括局部膜电容和离子迁移率。因此，介电泳力显微镜非常适合于生物系统的体外非接触扫描探针显微镜。
A novel scanning probe microscopy technique has allowed dielectrophoretic force imaging with nanoscale spatial resolution. Dielectrophoresis (DEP) traditionally describes the mobility of polarizable particles in inhomogeneous alternating current (ac) electric fields. Integrating DEP with atomic force microscopy allows for noncontact imaging with the image contrast related to the local electric polarizability. By tuning the ac frequency, dielectric spectroscopy can be performed at solid/liquid interfaces with high spatial resolution. In studies of cells, the frequency-dependent dielectrophoretic force is sensitive to biologically relevant electrical properties, including local membrane capacitance and ion mobility. Consequently, dielectrophoretic force microscopy is well suited for in vitro noncontact scanning probe microscopy of biological systems.