Epithelial to mesenchymal transition of mesothelial cells in tuberculous pleurisy.
Epithelial to mesenchymal transition of mesothelial cells in tuberculous pleurisy.
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DOI：
10.3349/ymj.2011.52.1.51
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发表时间：
2011-01
影响因子：
2.4
通讯作者：
Jang YS
中科院分区：
文献类型：
--
作者：
Kim C;Kim DG;Park SH;Hwang YI;Jang SH;Kim CH;Jung KS;Min K;Lee JW;Jang YS
结核性胸膜炎是肺结核最常见的肺外表现。尽管有适当的治疗，胸膜纤维化是一种常见的并发症，但其机制尚未阐明。本研究旨在确定结核性胸膜炎中间皮细胞是否发生上皮向间质转化（EMT）。用免疫荧光和逆转录聚合酶链反应（RT-PCR）对原发性自发性气胸手术中从灌洗液中分离出的正常胸膜间皮细胞进行了表征。这些细胞在体外用结核性胸膜炎流出液中产生的各种细胞因子处理。采用免疫荧光和RT-PCR方法对结核性胸膜炎流出液分离细胞进行分析。原发性自发性气胸灌洗液中分离的细胞具有上皮特征。这些细胞经过转化生长因子-β1和/或白细胞介素-1β处理后，表型从上皮细胞转变为间充质细胞，上皮细胞形态丧失，细胞角蛋白和e -钙粘蛋白表达减少。利用免疫荧光和RT-PCR对结核性胸膜炎的流出物进行分析，发现两种表型显示出间质特征和上皮和间质特征。我们的研究结果表明结核性胸膜炎的胸膜间皮细胞通过EMT参与了胸膜纤维化。
Tuberculous pleurisy is the most frequent extrapulmonary manifestation of tuberculosis. In spite of adequate treatment, pleural fibrosis is a common complication, but the mechanism has not been elucidated. This study is to determine whether epithelial to mesenchymal transition (EMT) of mesothelial cells occurs in tuberculous pleurisy. Normal pleural mesothelial cells, isolated from irrigation fluids during operations for primary spontaneous pneumothorax, were characterized by immunofluorescence and reverse transcription polymerase chain reaction (RT-PCR). These cells were treated in vitro with various cytokines, which were produced in the effluents of tuberculous pleurisy. The isolated cells from the effluents of tuberculous pleurisy were analyzed by immunofluorescence and RT-PCR analysis. The isolated cells from the irrigation fluid of primary spontaneous pneumothorax had epithelial characteristics. These cells, with transforming growth factor-β1 and/or interleukin-1β treatment, underwent phenotypic transition from epithelial to mesenchymal cells, with the loss of epithelial morphology and reduction in cytokeratin and E-cadherin expression. Effluent analysis from tuberculous pleurisy using immunofluorescence and RT-PCR demonstrated two phenotypes that showed mesenchymal characteristics and both epithelial & mesencymal characteristics. Our results suggest that pleural mesothelial cells in tuberculous pleurisy have been implicated in pleural fibrosis through EMT.