DIFFERENTIAL DOUBLE PULSE VOLTAMMETRY AT CHEMICALLY MODIFIED PLATINUM-ELECTRODES FOR INVIVO DETERMINATION OF CATECHOLAMINES
DIFFERENTIAL DOUBLE PULSE VOLTAMMETRY AT CHEMICALLY MODIFIED PLATINUM-ELECTRODES FOR INVIVO DETERMINATION OF CATECHOLAMINES
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DOI：
10.1021/ac50003a009
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发表时间：
1976-01-01
影响因子：
7.4
通讯作者：
HUBBARD, AT
中科院分区：
文献类型：
--
作者：
LANE, RF;HUBBARD, AT
Pt电极上薄膜形成的问题阻碍了微分脉冲伏安法在生理介质中对儿茶酚胺的分析测定。一种脉冲电化学技术，差分双脉冲伏安法，利用两个同时变化的不等方波电位脉冲作为线性直流斜坡的替代，并允许避免这些问题。理论和实验都很好地符合可逆的，2电子氧化到邻醌，没有进一步的并发症从均相化学反应表明。用I-水溶液对Pt表面进行化学修饰，提供了一种在感兴趣的电位范围内基本上没有电化学和化学干扰的电极。修饰电极对儿茶酚胺表现出未反应电极无法达到的敏感性。讨论了该方法对脑组织中多巴胺和去甲肾上腺素测定的潜在有用性。
Problems with film formation on Pt electrodes preclude the use of differential pulse voltammetry for analytical determination of catecholamines in physiological media. A pulse electrochemical technique, differential double pulse voltammetry, utilizes 2 simultaneously varying unequal square wave potential pulses as an alternative to the linear DC ramp and allows these problems to be avoided. Theory and experiment were in good agreement with a reversible, 2-electron oxidation to the o-quinones, with no further complications from homogeneous chemical reactions indicated. Chemical modification of Pt surfaces with aqueous I- provided an electrode which was essentially devoid of electrochemical and chemical interference over the potential range of interest. The modified electrodes exhibited a sensitivity toward the catecholamines unachievable with unreacted electrodes. The methodology was discussed in regard to its potential usefulness for the determination of dopamine and norepinephrine in brain tissue.