Localization of corin and atrial natriuretic peptide expression in human renal segments.
Localization of corin and atrial natriuretic peptide expression in human renal segments.
复制标题
Corin 和心钠素在人肾段表达的定位
DOI：
10.1042/cs20160398
复制
发表时间：
2016-09-01
期刊：
影响因子：
--
通讯作者：
Wu Q
中科院分区：
文献类型：
--
作者：
Dong L;Wang H;Dong N;Zhang C;Xue B;Wu Q
心钠素(ANP)介导的利钠反应是一种公认的心脏内分泌功能。Corin是一种跨膜蛋白水解酶，可以激活心脏中的心钠素。已经在非心脏组织中检测到Corin的表达，包括肾脏。在这里，我们检测了Corin，Pro-ANP/ANP和钠尿肽受体-A(NPR-A)在人肾段的表达。通过免疫组织化学染色和原位杂交，我们发现在人肾节段中有相似的Corin、Pro-ANP/ANP和NPR-A蛋白和mRNA的表达。在近曲小管和髓质连接管中表达最丰富。在近端小管中，Corin蛋白存在于刷状缘下的顶膜区，那里有丰富的ANP降解酶neprillysin。这些结果表明，Corin介导的前ANP激活可能发生在肾段，局部产生的ANP可能以自分泌的方式在原位调节钠和水的重吸收。我们的结果还表明，近曲小管是局部ANP作用的主要部位。在生理和病理条件下，这种肾脏Corin/ANP自分泌机制可能与心脏Corin/ANP内分泌机制在调节钠稳态方面有所不同。
Atrial natriuretic peptide (ANP)-mediated natriuretic response is a well-established cardiac endocrine function. Corin is a transmembrane protease that activates ANP in the heart. Corin expression has been detected in non-cardiac tissues including the kidney. Here we examined corin, pro-ANP/ANP and natriuretic peptide receptor-A (NPR-A) expression in human renal segments. By immunostaining and in situ hybridization, we found similar corin, pro-ANP/ANP and NPR-A protein and mRNA expression in human renal segments. The expression was most abundant in the proximal convoluted tubules and the medullary connecting ducts. In the proximal tubules, corin protein was present in the apical membrane region underneath the brush border where the ANP-degrading protease neprilysin was abundant. These results suggest that corin-mediated pro-ANP activation may occur in renal segments and that locally produced ANP may act in an autocrine manner to regulate sodium and water reabsorption in situ Our results also point to the proximal convoluted tubules as a major site for local ANP action. Such a renal corin/ANP autocrine mechanism may differ from the cardiac corin/ANP endocrine mechanism in regulating sodium homoeostasis under physiological and pathological conditions.