On the Determination of Lipolytic Activity of Lipase by Surfactant Non-addition Method
On the Determination of Lipolytic Activity of Lipase by Surfactant Non-addition Method
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不添加表面活性剂法测定脂肪酶的脂解活性
DOI：
10.5650/jos1956.36.638
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
T. Yamane
中科院分区：
文献类型：
--
作者：
T. Yamane
在通过表面活性剂非添加法测定脂肪酶的脂解活性时，尝试扩大滴定体积与添加到测定混合物中的酶量之间的比例范围，并减少重复测量数据（精密度）的变化。在反应容器中引入挡板在这两个方面都提供了一些改进。将搅拌从500 rpm增加到1000 rpm也可有效提高比例。
In the determination of the lipolytic activity of lipase by surfactant non-addition method, attempts were made to broaden the range of proportionality between the volume of titration and amount of enzyme added to the assay mixture and to decrease the variation in replicate measurement data (precision). The introduction of baffle plates into the reaction vessel provided some improvement in both these respects. Increased agitation from 500rpm to 1000rpm was also effective for greater proportionality.