Microbiologic analysis of periodontal pockets and carotid atheromatous plaques in advanced chronic periodontitis patients
Microbiologic analysis of periodontal pockets and carotid atheromatous plaques in advanced chronic periodontitis patients
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DOI：
10.1902/jop.2007.060473
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发表时间：
2007-09-01
影响因子：
4.3
通讯作者：
Nessi, Franco
中科院分区：
文献类型：
--
作者：
Aimetti, Mario;Romano, Federica;Nessi, Franco
背景：近年来，牙周致病菌在动脉粥样硬化斑块中定植的能力已成为研究者关注的焦点。然而，动脉粥样硬化和龈下菌斑样本中牙周致病细菌DNA的检出率之间的明确相关性尚未建立。我们的研究的目的是评估存在的五个牙周病原体（放线放线杆菌放线菌comitans，中间普氏菌，牙龈卟啉单胞菌，密螺旋体denticola，和Tannerella lasthia [原Tannerella lasthensis]）在牙周袋和颈动脉粥样硬化恢复从相同的patients.Methods：33例晚期慢性牙周炎患者进行动脉内膜切除术被列入研究。从龈下菌斑样本和颈动脉粥样硬化中提取DNA。结果：所有龈下菌斑标本均检测出至少一种目标微生物，其中16S rRNA基因部分片段为阳性，而16S rRNA基因部分片段为阴性。T. P.gingualis、T. denticola、P.intermedia和A.伴放线菌分别为69.7%、63.6%、54.5%、45.4%和33.3%。33例动脉内膜切除标本中31例检出细菌DNA。然而，没有一个样本的DNA检测阳性牙周病原体。结论：牙周细菌的存在动脉粥样硬化斑块没有证实这项调查，因此，没有相关性可以得出牙周炎细菌和微生物参与动脉粥样硬化病变之间。
Background: In recent years, many researchers have focused their attention on the ability of periodontal pathogens to colonize atheromatous plaques. Nevertheless, a clear correlation between the detection rates of periodontopathic bacterial DNA in atheromas and in subgingival plaque samples has not been established. The aim of our study was to assess the presence of five periodontal pathogens (Actinobacillus actinomycetemcomitans, Preuotella intermedia, Porphyromonas gingiualis, Treponema denticola, and Tannerella forsythia [formerly Tannerella forsythensis]) in periodontal pockets and in carotid atheromas recovered from the same patients.Methods: Thirty-three patients with advanced chronic periodontitis scheduled for endarterectomy were enrolled in the study. DNA was extracted from subgingival plaque samples and carotid atheromas. Universal bacteria primers for general detection of bacteria and species-specific primers for detection of periodontal pathogens were used to amplify part of the 16S rRNA gene by polymerase chain reaction.Results: All subgingival plaque samples were positive for at least one target microorganism. The prevalence of T. forsythia, P. gingiualis, T. denticola, P. intermedia, and A. actinomycetemcomitans were 69.7%, 63.6%, 54.5%, 45.4%, and 33.3%, respectively. Bacterial DNA was detected in 31 out of 33 endarterectomy specimens. However, none of the samples tested positive for DNA from periodontal pathogens.Conclusion: The presence of periodontal bacteria in atheromatous plaques was not confirmed by this investigation; thus, no correlation could be drawn between periodontitis bacteria and microorganisms involved in the atherosclerotic lesions.