Coordinated progression through two subtranscriptomes underlies the tachyzoite cycle of Toxoplasma gondii.
Coordinated progression through two subtranscriptomes underlies the tachyzoite cycle of Toxoplasma gondii.
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DOI：
10.1371/journal.pone.0012354
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发表时间：
2010-08-26
期刊：
影响因子：
3.7
通讯作者：
White MW
中科院分区：
文献类型：
--
作者：
Behnke MS;Wootton JC;Lehmann MM;Radke JB;Lucas O;Nawas J;Sibley LD;White MW
顶复门寄生虫通过各种不寻常的过程复制，其中细胞组分和染色体周期的典型真核扩增与传播所必需的寄生虫特异性结构的生物合成相协调。在这里，我们描述了全球细胞周期的转录组的速殖子阶段的弓形虫。在分裂速殖子，超过三分之一的mRNA表现出显着的周期性的配置文件，其时间相关的生物合成事件，展开在女儿寄生虫形成。这2，833个mRNA具有双峰组织，峰值表达发生在两个转录波之一中，这两个转录波由胞质分裂后进入S期和细胞周期退出的过渡所限制。G1-亚转录组富含基础生物合成和代谢功能所需的基因，类似于大多数真核生物，而S/M-亚转录组的特征在于寄生虫成熟，特化细胞器发育和感染性子细胞排出的独特apicomplexan要求。24个AP 2转录因子形成一个系列，通过速殖子周期与连续的蛋白质表达的尖峰在相同的时间范围内，作为他们的mRNA模式，表明负责蛋白质递送的时间的机制可能是由AP 2域介导的不同的启动子识别特异性。在顶复门细胞周期中的每一个主要事件以及更多的从属行为的基础上，是寄生虫基因表达的动态变化。负责周期性基因表达时机的机制可能对寄生虫复制的效率至关重要，并可能提供干扰寄生虫生长的新途径。
Apicomplexan parasites replicate by varied and unusual processes where the typically eukaryotic expansion of cellular components and chromosome cycle are coordinated with the biosynthesis of parasite-specific structures essential for transmission. Here we describe the global cell cycle transcriptome of the tachyzoite stage of Toxoplasma gondii. In dividing tachyzoites, more than a third of the mRNAs exhibit significant cyclical profiles whose timing correlates with biosynthetic events that unfold during daughter parasite formation. These 2,833 mRNAs have a bimodal organization with peak expression occurring in one of two transcriptional waves that are bounded by the transition into S phase and cell cycle exit following cytokinesis. The G1-subtranscriptome is enriched for genes required for basal biosynthetic and metabolic functions, similar to most eukaryotes, while the S/M-subtranscriptome is characterized by the uniquely apicomplexan requirements of parasite maturation, development of specialized organelles, and egress of infectious daughter cells. Two dozen AP2 transcription factors form a series through the tachyzoite cycle with successive sharp peaks of protein expression in the same timeframes as their mRNA patterns, indicating that the mechanisms responsible for the timing of protein delivery might be mediated by AP2 domains with different promoter recognition specificities. Underlying each of the major events in apicomplexan cell cycles, and many more subordinate actions, are dynamic changes in parasite gene expression. The mechanisms responsible for cyclical gene expression timing are likely crucial to the efficiency of parasite replication and may provide new avenues for interfering with parasite growth.
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影响因子：
4.3
作者：
Altschul SF;Wootton JC;Zaslavsky E;Yu YK
通讯作者：
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影响因子：
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影响因子：
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作者：
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DOI：
10.1073/pnas.97.18.10101
发表时间：
2000-08-29
影响因子：
11.1
作者：
Alter, O;Brown, PO;Botstein, D
通讯作者：
Botstein, D