Melatonin improves inflammatory cytokine profiles in lung inflammation associated with sleep deprivation
Melatonin improves inflammatory cytokine profiles in lung inflammation associated with sleep deprivation
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DOI：
10.3892/mmr.2012.814
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发表时间：
2012-05-01
影响因子：
3.4
通讯作者：
Baik, Haing-Woon
中科院分区：
文献类型：
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作者：
Kim, Ju-Young;Lee, Yang-Deok;Baik, Haing-Woon
睡眠障碍已成为现代国家的一种流行行为，而且其患病率也在增加。即使是轻微的睡眠不足也与健康问题有关。尤其是患有肺部疾病的患者经常抱怨失眠。我们最近表明，睡眠剥夺 (SD) 会加剧现有的急性肺部炎症，而褪黑激素治疗可通过抗细胞凋亡和抗氧化作用来减轻炎症。为了强化我们之前的报告，本研究旨在评估 SD 小鼠急性肺部炎症中的促炎介质。此外，我们还研究了炎症细胞向肺部的浸润情况。将25只ICR小鼠分为5组（n=5/组）：对照、SD、脂多糖（LPS）、LPS+SD和LPS+SD+褪黑激素。 SD 小鼠在多平台水浴中被剥夺睡眠 96 小时。第2天给予LPS（5mg/kg）和褪黑激素（5mg/kg）。第3天处死小鼠，收集血清和支气管肺泡灌洗（B L）液。 LPS+SD组血清炎症细胞因子水平升高。 LPS + SD 组的 BAL 液中白细胞介素 6、肿瘤坏死因子 α 和干扰素 γ 水平也有所增加。褪黑激素减少血清和支气管肺泡灌洗液中的炎症介质。 LPS 和 LPS + SD 小鼠中白细胞的积累升高，然而，褪黑激素抑制炎症细胞的募集（p
Sleep disturbance has become an endemic behavior in modern countries, and its prevalence has also increased. Even a subtle sleep deficiency is related to health problems. Particularly, patients with pulmonary disease often complain of insomnia. We recently showed that sleep deprivation (SD) exacerbates existing acute lung inflammation, and that melatonin treatment attenuates it via anti-apoptotic and anti-oxidant action. In order to reinforce our previous report, the present study was designed to evaluate pro-inflammatory mediators in acute lung inflammation in SD mice. In addition, we investigated the infiltration of inflammatory cells into the lungs. Twenty-five ICR mice were divided into 5 groups (n=5/ group): control, SD, lipopolysaccharide (LPS), LPS + SD and LPS + SD + melatonin. The SD mice were deprived of sleep for 96 h in a multiplatform water bath. LPS (5 mg/kg) and melatonin (5 mg/kg) were administered on day 2. The mice were sacrificed on day 3, and serum and bronchoalveolar lavage (BA L) fluid were collected. The serum levels of inflammatory cytokines were increased in the LPS + SD group. Interleukin-6, tumor necrosis factor-alpha and interferon-gamma levels were also increased in BAL fluid in the LPS + SD group. Melatonin reduced inflammatory mediators in the serum and BAL fluid. The accumulation of leukocytes in the LPS and LPS + SD mice was elevated, however, melatonin inhibited the recruitment of inflammatory cells (p