Polyaniline protected gold nanoparticles based mediator and label free electrochemical cortisol biosensor
Polyaniline protected gold nanoparticles based mediator and label free electrochemical cortisol biosensor
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DOI：
10.1016/j.bios.2011.07.015
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发表时间：
2011-10-15
影响因子：
12.6
通讯作者：
Bhansali, Shekhar
中科院分区：
文献类型：
--
作者：
Arya, Sunil K.;Dey, Abhishek;Bhansali, Shekhar
将聚苯胺保护的金纳米粒子（PPAuNPs）电沉积在金电极上，制备了电化学皮质醇生物传感器。皮质醇特异性单克隆抗体（C-Mab）使用N-乙基-N '-（3-二甲基氨基丙基）碳二亚胺和N-羟基琥珀酰亚胺（EDC/NHS）化学法共价固定到PPAuNP/Au电极的表面上。BSA用于阻断电极表面的非特异性吸附。采用透射电子显微镜、原子力显微镜和电化学阻抗技术分别对PPAuNP的形成和BSA/C-Mab/PPAuNP/Au电极的制备进行了表征。采用循环伏安法和微分脉冲伏安法测定了磷酸盐缓冲液（PBS）中皮质醇的浓度。结果证实，基于PPAuNP的电极在重复扫描期间是稳定的，并且表现出可重复的氧化还原峰。此外，PBS缓冲液中的BSA/C-Mab/PPAuNP/Au电极以1.63 μ A M（-1）的灵敏度准确地检测1 pM-100 nM范围内的皮质醇。该生物传感器对BSA和17-α-羟基孕酮具有选择性。本研究建立了使用PPAuNP为基础的矩阵的标记和介体自由的电化学生物传感器皮质醇，应激生物标志物的可行性。(C)2011 Elsevier B. V.保留所有权利。
Polyaniline protected gold nanoparticles (PPAuNPs) were electrophoretically deposited onto a gold electrode, and utilized to fabricate an electrochemical cortisol biosensor. Cortisol specific monoclonal antibody (C-Mab) was covalently immobilized onto the surface of a PPAuNP/Au electrode using N-ethyl-N'-(3-dimethylaminopropyl) carbodiimide and N-hydroxysuccinimide (EDC/NHS) chemistry. BSA was employed for blocking nonspecific adsorption on the electrode surface. PPAuNP formation and BSA/C-Mab/PPAuNP/Au electrode fabrication were characterized using transmission electron microscopy, atomic force microscopy and electrochemical impedance techniques, respectively. Cyclic voltammetry and differential pulse voltammetric techniques were used to determine the cortisol concentration in a phosphate buffer saline (PBS) solution. Results confirmed that the PPAuNP based electrode was stable during repeated scans and exhibited repeatable redox peaks. Further, the BSA/C-Mab/PPAuNP/Au electrode in the PBS buffer accurately detected cortisol in the range of 1 pM-100 nM with a sensitivity of 1.63 mu A M(-1). The biosensor was found to be selective against BSA and 17-alpha-hydroxy progesterone. This research establishes the feasibility of using a PPAuNP based matrix for a label and mediator free electrochemical biosensor for cortisol, a stress biomarker. (C) 2011 Elsevier B.V. All rights reserved.