Using Diatom and Apicomplexan Models to Study the Heme Pathway of Chromera velia.
Using Diatom and Apicomplexan Models to Study the Heme Pathway of Chromera velia.
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DOI：
10.3390/ijms22126495
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发表时间：
2021-06-17
影响因子：
5.6
通讯作者：
Oborník M
中科院分区：
文献类型：
--
作者：
Richtová J;Sheiner L;Gruber A;Yang SM;Kořený L;Striepen B;Oborník M
血红素的生物合成对几乎所有生物体都是必不可少的。尽管其功能保守，但该途径的酶可以位于不同生物体中不同的细胞区室中。这个位置并不总是反映它们的进化起源，正如它们通过内共生获得的历史所期望的那样。相反，酶的最终亚细胞定位反映了多种因素，包括进化起源、对产物的需求、底物的可用性和途径调节机制。顶单胞体中血红素的生物合成遵循一个嵌合途径，将来自古代藻类共生体和宿主的血红素元素结合在一起。计算分析使用不同的算法预测复杂的靶向模式，将酶放置在线粒体、质体、内质网或细胞质中。我们利用异源报告基因在顶复合体寄生虫刚地弓形虫和硅藻三角藻中的表达来实验验证这些预测。在两种转染系统中，5-氨基乙酰丙酸合成酶都位于线粒体中。弓形虫的两种5-氨基乙酰酸脱水酶位于细胞质中，两种铁螯合酶位于线粒体中，两种铁螯合酶位于质体中。在三角藻中，所有剩余的酶，从ala脱水酶到铁螯合酶，都被放置在内质网或质体周围空间。
Heme biosynthesis is essential for almost all living organisms. Despite its conserved function, the pathway’s enzymes can be located in a remarkable diversity of cellular compartments in different organisms. This location does not always reflect their evolutionary origins, as might be expected from the history of their acquisition through endosymbiosis. Instead, the final subcellular localization of the enzyme reflects multiple factors, including evolutionary origin, demand for the product, availability of the substrate, and mechanism of pathway regulation. The biosynthesis of heme in the apicomonad Chromera velia follows a chimeric pathway combining heme elements from the ancient algal symbiont and the host. Computational analyses using different algorithms predict complex targeting patterns, placing enzymes in the mitochondrion, plastid, endoplasmic reticulum, or the cytoplasm. We employed heterologous reporter gene expression in the apicomplexan parasite Toxoplasma gondii and the diatom Phaeodactylum tricornutum to experimentally test these predictions. 5-aminolevulinate synthase was located in the mitochondria in both transfection systems. In T. gondii, the two 5-aminolevulinate dehydratases were located in the cytosol, uroporphyrinogen synthase in the mitochondrion, and the two ferrochelatases in the plastid. In P. tricornutum, all remaining enzymes, from ALA-dehydratase to ferrochelatase, were placed either in the endoplasmic reticulum or in the periplastidial space.
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影响因子：
2.5
作者：
Fussy, Zoltan;Masarova, Petra;Obornik, Miroslav
通讯作者：
Obornik, Miroslav
影响因子：
4.8
作者：
Agrawal, Swati;van Dooren, Giel G.;Striepen, Boris
通讯作者：
Striepen, Boris
DOI：
10.1074/mcp.m114.043083
发表时间：
2015-04
期刊：
Molecular & cellular proteomics : MCP
影响因子：
--
作者：
Fukasawa Y;Tsuji J;Fu SC;Tomii K;Horton P;Imai K
通讯作者：
Imai K
影响因子：
5.6
作者：
Emanuelsson, O;Nielsen, H;von Heijne, G
通讯作者：
von Heijne, G
DOI：
10.1007/bf02172403
发表时间：
1996-10-16
期刊：
MOLECULAR & GENERAL GENETICS
影响因子：
--
作者：
Apt, KE;KrothPancic, PG;Grossman, AR
通讯作者：
Grossman, AR