Studies on the relative biopotencies and intestinal absorption of different apo-beta-carotenoids in rats and chickens.
Studies on the relative biopotencies and intestinal absorption of different apo-beta-carotenoids in rats and chickens.
复制标题
不同apo-β-类胡萝卜素在大鼠和鸡体内的相对生物效能和肠道吸收的研究。
作者：
R. V. Sharma;S. Mathur;J. Ganguly
1. 通过疗效生长测定和肝脏储存试验，以摩尔为基础，在大鼠中研究了几种类胡萝卜素（例如 8'-、10'- 和 12'-apo-β-胡萝卜醛以及 8'-apo-β-胡萝卜酸甲酯）相对于 β-胡萝卜素的生物效能。 2. 在治疗性生长测定中，以摩尔计，8'-、10'-和 12'-apo-β-胡萝卜醛以及 8'-apo-β-胡萝卜酸甲酯的生物效价分别为 72%、78%、72% 和 53%，而以重量计，相应的值为 93%、111%、111% 和 63%。 β-胡萝卜素取100%。就维生素 A 的产量而言，肝脏储存测试中的这些值要低得多。 3.当喂食8'-apo-β-胡萝卜素时，未变化的醛与少量相应的醇和较大比例的酸一起迅速出现在大鼠和鸡的组织中。 8'-阿朴胡萝卜素醇、8'-阿朴胡萝卜素酸及其甲酯被吸收且未变化。游离酸从组织中消失得最快，但其甲酯在组织中存留时间最长。 4.根据这些观察结果表明，大部分脱胡萝卜醛被氧化成相应的酸，而相应的酸又大部分降解成视黄酸，其中小部分被双加氧酶系统攻击，产生视黄醛。
1. The biopotencies relative to beta-carotene of several apocarotenoids, such as 8'-, 10'- and 12'-apo-beta-carotenal and methyl 8'-apo-beta-carotenoate, were investigated in rats, on a molar basis, by both curative-growth assay and liver-storage tests. 2. In the curative-growth assays, on a molar basis the biopotencies of 8'-, 10'- and 12'-apo-beta-carotenal and methyl 8'-apo-beta-carotenoate were 72, 78, 72 and 53% respectively, whereas on a weight basis the corresponding values were 93, 111, 111 and 63%, with respect to beta-carotene taken as 100%. In terms of yield of vitamin A, these values were much lower in the liver-storage tests. 3. When 8'-apo-beta-carotenal was fed, the unchanged aldehyde together with small amounts of the corresponding alcohol and larger proportions of the acid rapidly appeared in the tissues of both rats and chickens. The 8'-apocarotenol, 8'-apocarotenoic acid and its methyl ester were absorbed unchanged. The free acid disappeared most rapidly from the tissues, but its methyl ester persisted in the tissues longest. 4. On the basis of these observations it is suggested that most of an apocarotenal is oxidized to the corresponding acid, which, in turn, is mostly degraded to retinoic acid, with small proportions of it being attacked by the dioxygenase system giving retinal.