Genetic diversity and population structure of genes encoding vaccine candidate antigens of Plasmodium vivax
Genetic diversity and population structure of genes encoding vaccine candidate antigens of Plasmodium vivax
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DOI：
10.1186/1475-2875-11-68
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发表时间：
2012-03-14
期刊：
影响因子：
3
通讯作者：
Bacon, David J.
中科院分区：
文献类型：
--
作者：
Chenet, Stella M.;Tapia, Lorena L.;Bacon, David J.
背景资料：疟疾疫苗开发中的一个主要问题是在世界各地不同地理区域的疟原虫分离株中通常观察到的遗传多态性。恶性疟原虫和间日疟原虫抗原性表面蛋白如环子孢子蛋白（CSP）、Duffy结合蛋白（DBP）、裂殖子表面蛋白-1（MSP-1）、顶膜抗原-1（AMA-1）和血小板反应蛋白相关无名蛋白（TRAP）中已观察到高度多态性区域。在来自秘鲁的洛雷托亚马逊地区的106个分离株中，评估了间日疟原虫中各种疫苗候选抗原的重要多态性区域的遗传变异性。此外，在秘鲁分离株确定的遗传多样性进行了比较，从不同的地理位置world.Results：在间日疟原虫种群中发现的结构多样性没有显示出地理模式和单倍型从所有候选基因分布在世界各地的人口研究。此外，平衡选择的证据被发现在多态性区域的陷阱，DBP和AMA-1 genes.Conclusions：重要的是要有一个很好的代表性的单倍型在世界范围内流通时，实施疫苗，无论地理区域的部署，因为选择压力结构抗原多样性中起着重要的作用。
Background: A major concern in malaria vaccine development is genetic polymorphisms typically observed among Plasmodium isolates in different geographical areas across the world. Highly polymorphic regions have been observed in Plasmodium falciparum and Plasmodium vivax antigenic surface proteins such as Circumsporozoite protein (CSP), Duffy-binding protein (DBP), Merozoite surface protein-1 (MSP-1), Apical membrane antigen-1 (AMA-1) and Thrombospondin related anonymous protein (TRAP).Methods: Genetic variability was assessed in important polymorphic regions of various vaccine candidate antigens in P. vivax among 106 isolates from the Amazon Region of Loreto, Peru. In addition, genetic diversity determined in Peruvian isolates was compared to population studies from various geographical locations worldwide.Results: The structured diversity found in P. vivax populations did not show a geographic pattern and haplotypes from all gene candidates were distributed worldwide. In addition, evidence of balancing selection was found in polymorphic regions of the trap, dbp and ama-1 genes.Conclusions: It is important to have a good representation of the haplotypes circulating worldwide when implementing a vaccine, regardless of the geographic region of deployment since selective pressure plays an important role in structuring antigen diversity.