A Cell Phone-Based Microphotometric System for Rapid Antimicrobial Susceptibility Testing
A Cell Phone-Based Microphotometric System for Rapid Antimicrobial Susceptibility Testing
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DOI：
10.1177/2211068213491095
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发表时间：
2014-06-01
期刊：
影响因子：
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通讯作者：
Wong, Pak Kin
中科院分区：
文献类型：
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作者：
Kadlec, Meichei Wang;You, David;Wong, Pak Kin
本研究展示了一种低成本、便携式的诊断系统，用于在资源有限的环境中进行快速抗菌药物敏感性测试。为了确定抗微生物剂抗性表型，在不同抗生素条件下检测微孔阵列中病原体的生长。与标准吸收光谱法相比，使用比色细胞活力试剂显着提高了测定的灵敏度。气体可渗透的微孔阵列被并入以促进快速细菌生长并消除对笨重的支持设备的需求。抗生素也可以预涂覆在微孔阵列中，以简化针对护理点应用的测定方案。此外，一个低成本的手机为基础的显微光度系统，用于检测在微孔阵列中的细菌生长。通过优化操作条件，该系统允许对初始浓度为10（1）至10（6）cfu/mL的样品进行抗菌药物敏感性测试。使用尿路感染作为模型系统，我们展示了培养基和尿液中尿路病原体的快速抗菌药物耐药性谱。由于其简单性和成本效益，基于手机的显微光度测量系统预计将在资源有限的环境中具有广泛的适用性，以管理由多药耐药病原体引起的传染病。
This study demonstrates a low-cost, portable diagnostic system for rapid antimicrobial susceptibility testing in resource-limited settings. To determine the antimicrobial resistance phenotypically, the growth of pathogens in microwell arrays is detected under different antibiotic conditions. The use of a colorimetric cell viability reagent is shown to significantly improve the sensitivity of the assay compared with standard absorbance spectroscopy. Gas-permeable microwell arrays are incorporated for facilitating rapid bacterial growth and eliminating the requirement of bulky supporting equipment. Antibiotics can also be precoated in the microwell array to simplify the assay protocol toward point-of-care applications. Furthermore, a low-cost cell phone-based microphotometric system is developed for detecting the bacterial growth in the microwell array. By optimizing the operating conditions, the system allows antimicrobial susceptibility testing for samples with initial concentrations from 10(1) to 10(6) cfu/mL. Using urinary tract infection as the model system, we demonstrate rapid antimicrobial resistance profiling for uropathogens in both culture media and urine. With its simplicity and cost-effectiveness, the cell phone-based microphotometric system is anticipated to have broad applicability in resource-limited settings toward the management of infectious diseases caused by multidrug-resistant pathogens.