The use of debrided human articular cartilage for autologous chondrocyte implantation: maintenance of chondrocyte differentiation and proliferation in type I collagen gels
The use of debrided human articular cartilage for autologous chondrocyte implantation: maintenance of chondrocyte differentiation and proliferation in type I collagen gels
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DOI：
10.1016/j.orthres.2003.07.001
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发表时间：
2004-03-01
影响因子：
2.8
通讯作者：
Van Damme, MPI
中科院分区：
文献类型：
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作者：
Chaipinyo, K;Oakes, BW;Van Damme, MPI
自体软骨细胞植入 (ACI) 是治疗大面积全层膝关节关节软骨 (AC) 缺损的最有前途的手术治疗方法，其中来自健康非承重区域 AC 的细胞在体外繁殖并植入此类缺损中。在针对有症状的膝部全层 AC 缺损的常规手术中，通常会清创并丢弃此类缺损边缘和基部周围受损的 AC。本研究的目的是检查来自这种“清创”AC 的软骨细胞是否可以增殖、合成软骨特异性基质，从而可用于 ACI。方法：从 12 名患者（清创关节软骨：DAC，年龄 35-61 岁）和两例尸检（正常关节软骨：NAC，年龄 21 岁和 25 岁）中获取活组织检查。分离软骨细胞，以低密度接种在 I 型胶原凝胶中，并作为单层培养物培养 4 周，无需传代。结果：在 I 型胶原凝胶中培养 4 周后，DAC 的细胞增殖（18.34 +/- 1.95 倍）与 NAC 的细胞增殖（11.24 +/- 1.02 倍）相似。 DAC 中蛋白多糖和胶原蛋白的合成也与 NAC 相似。免疫标记和 SDS-PAGE 及荧光照相显示，get 培养物中新合成的基质主要由 II 型胶原组成。来自在 I 型胶原凝胶中低密度培养的“清创人 AC”的软骨细胞可用于 ACI 程序，因为它们为 ACI 提供足够的活细胞数量，并在合成软骨样基质时保持其软骨细胞表型。 (C) 2003 年骨科研究学会。由爱思唯尔有限公司出版。保留所有权利。
Autologous chondrocyte implantation (ACI) is the most promising surgical treatment for large full thickness knee joint articular cartilage (AC) defects where cells from healthy non-weight bearing area AC are multiplied in vitro and implanted into such defects. In the routine Surgical procedure for symptomatic knee full thickness AC defects, damaged AC surrounding the edge and the base of such defects is usually debrided and discarded. The purpose of this study was to examine if chondrocytes from this 'debrided' AC can proliferate, synthesize a cartilage specific matrix and thus can be used for ACI. Methods: Biopsies were retrieved from 12 patients (debrided articular cartilage: DAC, aged 35-61) and from two autopsies (normal articular cartilage: NAC, aged 21 and 25). Chondrocytes were isolated, seeded at low density in type I collagen gels and as monolayer cultures for 4 weeks without passage.Results: After 4 weeks cultures in type I collagen gels, cell proliferation from DAC (18.34 +/- 1.95 fold) was similar to cells from NAC (11.24 +/- 1.02 fold). Syntheses of proteoglycan and collagen in DAC were also similar to NAC. Newly synthesized matrices in get cultures consisted predominantly of type II collagen as shown by immuno-labelling and SDS-PAGE followed by fluorography. Chondrocytes from 'debrided human AC' cultured at low density in type I collagen gels may be used for the ACI procedure as they provide sufficient viable cell numbers for ACI and maintain their chondrocyte phenotype as they synthesize a cartilage-like matrix. (C) 2003 Orthopaedic Research Society. Published by Elsevier Ltd. All rights reserved.