Genetic Components of Root Architecture Remodeling in Response to Salt Stress
Genetic Components of Root Architecture Remodeling in Response to Salt Stress
复制标题
盐胁迫下根构型重塑的遗传成分
DOI：
10.1105/tpc.16.00680
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发表时间：
2017-12-01
期刊：
影响因子：
11.6
通讯作者：
Testerink, Christa
中科院分区：
文献类型：
--
作者：
Julkowska, Magdalena M.;Koevoets, Iko T.;Testerink, Christa
土壤中的盐分对植物的生长极为有害。植物通过主根和侧根之间的根质量的重新分配来响应，然而这一过程背后的遗传机制仍然是未知的。在这里，我们描述了347拟南芥加入根系统结构（RSA）的自然变异，并确定了最高的自然变异，在他们的响应盐的性状。使用全基因组关联研究，盐诱导的RSA变化与100个遗传位点相关。两个候选位点与侧根发育进行了验证和进一步调查。CYP79B2在盐胁迫下的表达变化与侧根的发育呈正相关，CYP79B2和CYP79B3双突变体在盐胁迫下侧根发育较少且较短，但在对照条件下不发育侧根。相比之下，高HKT1表达的根抑制侧根的发展，这可能是部分获救的钾。通过Salt_NV_Root应用程序收集的数据和多个RSA性状的多变量分析可捕获根系对盐度的反应。总之，我们的研究结果提供了一个更好的理解有效的RSA重塑反应，以及所涉及的遗传成分，植物在胁迫条件下的性能。
Salinity of the soil is highly detrimental to plant growth. Plants respond by a redistribution of root mass between main and lateral roots, yet the genetic machinery underlying this process is still largely unknown. Here, we describe the natural variation among 347 Arabidopsis thaliana accessions in root system architecture (RSA) and identify the traits with highest natural variation in their response to salt. Salt-induced changes in RSA were associated with 100 genetic loci using genome-wide association studies. Two candidate loci associated with lateral root development were validated and further investigated. Changes in CYP79B2 expression in salt stress positively correlated with lateral root development in accessions, and cyp79b2 cyp79b3 double mutants developed fewer and shorter lateral roots under salt stress, but not in control conditions. By contrast, high HKT1 expression in the root repressed lateral root development, which could be partially rescued by addition of potassium. The collected data and multivariate analysis of multiple RSA traits, available through the Salt_NV_Root App, capture root responses to salinity. Together, our results provide a better understanding of effective RSA remodeling responses, and the genetic components involved, for plant performance in stress conditions.