Evidence for repair in trypan blue-treated mouse egg cylinders: an electron microscopic study.
Evidence for repair in trypan blue-treated mouse egg cylinders: an electron microscopic study.
复制标题
台盼蓝处理的小鼠卵缸修复的证据：电子显微镜研究。
DOI：
10.1002/tera.1420290310
复制
发表时间：
1984
期刊：
影响因子：
--
通讯作者：
Mendoza,LA
中科院分区：
文献类型：
--
作者：
Hamburgh,M;Glezer,I;Sabater,J;Louis-JacquesJr,G;Francois,M;Mendoza,LA
哺乳动物胎儿是否能纠正胚胎发育早期致畸剂引起的损伤的问题被重新研究。为此，在妊娠第6天给雌性小鼠注射致畸染料台盼蓝。在6，24，和48小时内暴露于致畸剂，卵筒被删除，切片，并准备电子显微镜分析。检查了以下细胞器：（1）线粒体，（2）高尔基体，（3）内质网和（4）游离多核糖体。在超微结构水平上，暴露于台盼蓝导致6小时内的内质网的碎片和消耗的游离多聚核糖体的内胚层的卵圆筒。在外胚层细胞中，只有多聚核糖体的分布受到干扰后，暴露于台盼蓝。注射台盼蓝后24小时收获的卵柱部分恢复。它们的内质网和多核糖体似乎更接近对照组。注射后48小时获得的大多数卵圆柱体的两个胚层的细胞在用电子显微镜观察时与对照没有区别。台盼蓝处理后，线粒体或高尔基体没有发现一致的变化。它的结论是，小鼠胚胎似乎能够纠正损害持续在卵缸阶段，尽管早期损伤影响两个胚层，这样的卵缸可以正常发育，如显微镜检查所示。
The question of whether mammalian fetuses can correct damage induced by teratogens during early stages of embryogenesis was reinvestigated. Toward this purpose female mice were injected on day 6 of gestation with the teratogenic dye trypan blue. Within 6, 24, and 48 hr of exposure to the teratogen, egg cylinders were removed, sectioned, and prepared for electron microscopic analysis. The following organelles were examined: (1) mitochondria, (2) Golgi apparatus, (3) endoplasmic reticulum, and (4) free polysomes. On the ultrastructural level, exposure to trypan blue lead within 6 hr to fragmentation of the endoplasmic reticulum and a depletion of free polysomes in the endoderm of the egg cylinders. In ectodermal cells only the distribution of polysomes was disturbed following exposure to trypan blue. Egg cylinders harvested 24 hr after injection of trypan blue had partially recovered. Their endoplasmic reticulum and polysomes appeared closer to controls. The cells of both germ layers of most egg cylinders obtained 48 hr after injection were indistinguishable from controls when viewed with the electron microscope. No consistent changes were found in mitochondria or Golgi apparatus following trypan blue treatment. It is concluded that mouse embryos appear to be able to correct damage sustained during the egg cylinder stage, and that in spite of earlier injury affecting both germ layers such egg cylinders can develop normally as revealed by microscopic examination.