Isolation, Sequence, Infectivity and Replication Kinetics of Severe Acute Respiratory Syndrome Coronavirus 2
Isolation, Sequence, Infectivity and Replication Kinetics of Severe Acute Respiratory Syndrome Coronavirus 2
复制标题
DOI：
10.3201/eid2609.201495
复制
发表时间：
2020-09-01
影响因子：
11.8
通讯作者：
Mubareka, Samira
中科院分区：
文献类型：
--
作者：
Banerjee, Arinjay;Nasir, Jalees A.;Mubareka, Samira
自2019年12月在中国武汉出现以来，严重急性呼吸综合征冠状病毒2（SARS-CoV-2）已感染全球约600万人。随着SARS-CoV-2在全球的传播，我们探索了可能被这种病毒感染的人类细胞的范围。我们从加拿大多伦多的2名感染患者中分离出SARS-CoV-2;确定了基因组序列;并在我们的病毒库存的代表性群体中鉴定了单核苷酸变化。我们还测试了广泛的人类免疫细胞对SARS-CoV-2的生产性感染。我们证实，人原代外周血单个核细胞是不允许的SARS-CoV-2。随着SARS-CoV-2继续在全球范围内传播，监测病毒中的单核苷酸多态性并继续分离循环病毒以通过使用体外和体内感染模型确定病毒基因型和表型至关重要。
Since its emergence in Wuhan, China, in December 2019, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has infected approximate to 6 million persons worldwide. As SARS-CoV-2 spreads across the planet, we explored the range of human cells that can be infected by this virus. We isolated SARS-CoV-2 from 2 infected patients in Toronto, Canada; determined the genomic sequences; and identified single-nucleotide changes in representative populations of our virus stocks. We also tested a wide range of human immune cells for productive infection with SARS-CoV-2. We confirm that human primary peripheral blood mononuclear cells are not permissive for SARS-CoV-2. As SARS-CoV-2 continues to spread globally, it is essential to monitor single-nucleotide polymorphisms in the virus and to continue to isolate circulating viruses to determine viral genotype and phenotype by using in vitro and in vivo infection models.