Distribution of the glucose transporter in the mammalian brain.
Distribution of the glucose transporter in the mammalian brain.
复制标题
葡萄糖转运蛋白在哺乳动物大脑中的分布。
DOI：
10.1111/j.1471-4159.1986.tb01755.x
复制
发表时间：
1986
影响因子：
4.7
通讯作者：
Harik,SI
中科院分区：
文献类型：
--
作者：
Dick,AP;Harik,SI
我们使用[3 H]细胞松弛素B作为特异性配体来研究以下组织制备物的葡萄糖转运蛋白：（a）来自大鼠和猪的大脑皮层和小脑的微血管，（B）大鼠和猪的大脑皮层和小脑的颗粒部分，（c）猪的侧脑室、第三和第四脑室脉络丛，以及（d）来自猪大脑皮层的突触体。[3 H]细胞松弛素B的特异性D-葡萄糖置换结合存在于所有研究的制剂中。这种结合是饱和的，并显示了一类结合位点的动力学，类似于在其他哺乳动物组织中发现的葡萄糖转运蛋白。葡萄糖转运蛋白的密度在大脑和小脑的微血管和脉络丛中比在大脑和小脑的粗颗粒部分或在大脑突触体中高得多。这些发现与脑微血管的生理功能相一致，脑微血管不仅运输葡萄糖，而且还运输整个大脑的更大质量。在猪中，大脑微血管中葡萄糖转运蛋白的密度显著高于小脑微血管。用[3 H]细胞松弛素B对突触体膜葡萄糖转运蛋白进行不可逆的光亲和标记，然后进行溶解和聚丙烯酰胺凝胶电泳，结果显示单个放射性区域对应于60，000 - 64，000道尔顿的分子量。
We used [3H]cytochalasin B as a specific ligand to study the glucose transporter of the following tissue preparations: (a) microvessels derived from the cerebral cortex and cerebellum of the rat and pig, (b) particulate fractions of the cerebral cortex and cerebellum of the rat and pig, (c) lateral, third, and fourth ventricular choroid plexus of the pig, and (d) synaptosomes from the pig cerebral cortex. Specific, D‐glucose‐displaceable binding of [3H]cytochalasin B was present in all the preparations studied. This binding was saturable and displayed the kinetics of a single class of binding sites, similar to the glucose transporter found in other mammalian tissues. The density of the glucose transporter was much higher in cerebral and cerebellar microvessels and choroid plexus than either in crude particulate fractions of the cerebrum and cerebellum or in cerebral synaptosomes. These findings agree with the physiologic function of brain microvessels that transport glucose, not only for their own use, but also for the much greater mass of the entire brain. In the pig, the density of the glucose transporter in cerebral microvessels was significantly higher than in cerebellar microvessels. Irreversible photoaffinity labeling of the glucose transporter of synaptosomal membranes with [3H]cytochalasin B followed by solubilization and polyacrylamide gel electrophoresis demonstrated a single region of radioactivity that corresponded to a molecular mass of 60,000–64,000 daltons.