Effects of cigarette smoke exposure and cessation on inflammatory cells and matrix metalloproteinase activity in mice
Effects of cigarette smoke exposure and cessation on inflammatory cells and matrix metalloproteinase activity in mice
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DOI：
10.1080/01902140490252858
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发表时间：
2004-01-01
影响因子：
1.7
通讯作者：
Nikula, KJ
中科院分区：
文献类型：
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作者：
Seagrave, J;Barr, EB;Nikula, KJ
B6C3F（1）雌性小鼠暴露于香烟烟雾（CS）（250 mg/m（3）总颗粒物质）或过滤空气（FA），每天6小时，每周5天，持续6、7或10周，或暴露于CS 6周，然后再暴露于FA 1或4周。暴露于CS会增加支气管肺泡灌洗液中巨噬细胞、中性粒细胞、淋巴细胞以及基质金属蛋白酶(MMP)-2和MMP-9的含量。停止接触 CS 后 1 周观察到大多数灌洗参数（淋巴细胞除外）部分恢复，4 周后进一步降低，但间质炎症持续时间更长。这些结果支持 MMP 在 CS 引起的肺气肿中的作用，并表明戒烟可以恢复正常的体内平衡。
B6C3F(1) female mice were exposed to cigarette smoke (CS) (250 mg/m(3) total particulate material) or filtered air (FA), 6 hours/day, 5 days/week, for 6, 7, or 10 weeks, or to CS for 6 weeks, then FA for 1 or 4 additional weeks. Exposure to CS increased macrophages, neutrophils, lymphocytes and matrix metalloproteinase (MMP)-2 and MMP-9 content in bronchoalveolar lavage fluid. Partial recovery of most lavage Parameters (except lymphocytes) was observed I week after cessation of CS exposure with further reductions after 4 weeks, but interstitial inflammation persisted longer. These results support a role for MMPs in CS-induced emphysema and indicate that smoking cessation allows restoration toward normal homeostasis.