Biosynthesis of acetate esters by dominate strains, isolated from Chinese traditional fermented fish (Suan yu)
Biosynthesis of acetate esters by dominate strains, isolated from Chinese traditional fermented fish (Suan yu)
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从中国传统发酵鱼（酸鱼）中分离的优势菌株生物合成醋酸酯
DOI：
10.1016/j.foodchem.2017.10.007
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发表时间：
2018-04-01
期刊：
影响因子：
8.8
通讯作者：
Xia, Wenshui
中科院分区：
文献类型：
--
作者：
Gao, Pei;Jiang, Qixing;Xia, Wenshui
研究了从中国传统发酵鱼中分离的植物乳杆菌120、木糖葡萄球菌135和酿酒酵母31合成乙酸酯的特性。建立了含有酰基供体（乙酸/三乙酸甘油酯/乙酰辅酶A）和脂肪醇（C2-C6）的缓冲体系，接种三株菌的胞内和胞外提取物。结果表明，L. plantarum 120的生物合成途径为酯化和醇解，而S. xylosus 135和S. cerevisiae 31是水解和酯化，而不是醇解。L. plantarum 120在高pH条件下醇解的产酶能力高于酯化，而在低pH条件下，两种菌株的产酶能力均相反。此外，L. plantarum 120比S. xylosus 135和S.酿酒酵母31.此外，随着脂肪醇碳数的增加，微生物酯合成活性也随之增加。
Biosynthesis of acetate esters by Lactobacillus plantarum 120, Staphylococcus xylosus 135 and Saccharomyces cerevisiae 31, isolated from Chinese traditional fermented fish (Suan yu) were studied. A buffer system containing acyl donors (acetic acid/glyceryl triacetate/acetyl-CoA) and aliphatic alcohols (C2-C6) was established, inoculated with intracellular and extracellular extracts of the three strains. The results showed that the biosynthesis pathway of L. plantarum 120 was esterification and alcoholysis, while the biosynthesis pathway of S. xylosus 135 and S. cerevisiae 31 was hydrolysis and esterification, rather than alcoholysis. The ester-synthesizing activity of L. plantarum 120 via alcoholysis was higher than that via esterification at high pH value, while an opposite result for each strain was observed at low pH value. Moreover, the ester-synthesis activity of L. plantarum 120 was higher than that of S. xylosus 135 and S. cerevisiae 31. In addition, microbial ester-synthesis activity increased with the increase of aliphatic alcohol carbon number.