Identification and characterization of the novel centrosomal protein centlein.
Identification and characterization of the novel centrosomal protein centlein.
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DOI：
10.1016/j.bbrc.2007.12.050
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发表时间：
2008-02
影响因子：
3.1
通讯作者：
K. Makino;Kazuaki Umeda;Akiyoshi Uezu;Yukari Hiragami;Tatsuhiko Sakamoto;H. Ihn;H. Nakanishi
中科院分区：
文献类型：
--
作者：
K. Makino;Kazuaki Umeda;Akiyoshi Uezu;Yukari Hiragami;Tatsuhiko Sakamoto;H. Ihn;H. Nakanishi
中心体作为主要微管（MT）组织中心发挥作用。它们由一对中心粒组成，中心粒被中心粒周物质包围。在这里，我们描述了一种新的蛋白质，命名为中心肽（中心体蛋白）的分子特征。中心肽是一种由721个氨基酸组成的蛋白质，计算分子量为82，717，具有卷曲螺旋结构域。Western blot分析表明，centlein是普遍表达。在分裂间期和有丝分裂期，内源性中心肽和增强型绿色荧光蛋白融合中心肽定位于中心体。当从用诺考达唑（一种MT破坏剂）处理的细胞中分离中心体时，中心肽和中心体蛋白γ-微管蛋白富集在相同的级分中。这些数据表明，中心肽是一个广泛的中心体蛋白，它与中心体的协会是独立的MT。中心肽在G1期富集在中心粒中，表明中心肽可能参与中心粒相关的功能，如中心粒的复制和初级纤毛的产生。
Centrosomes function as the major microtubule (MT)-organizing center. They are composed of a pair of centrioles which are surrounded by the pericentriolar material. Here, we describe the molecular characterization of a novel protein, named centlein (centrosomal protein). Centlein was a protein of 721 amino acids with a calculated molecular weight of 82,717 and possessed coiled-coil domains. Western blot analysis indicated that centlein was ubiquitously expressed. Endogenous centlein as well as enhanced green fluorescent protein-fused centlein was localized at centrosomes in interphase and mitosis. When centrosomes were isolated from cells treated with nocodazole, an MT-disrupting agent, centlein and the centrosomal protein, γ-tubulin, were enriched in the same fractions. These data indicate that centlein is a widespread centrosomal protein and that its association with centrosomes is independent of MTs. Centlein appeared to be enriched in the mother centriole in G1 phase, suggesting possible involvement of centlein in mother-centriole-related functions, such as duplication of centrioles and generation of primary cilia.