Prognostic relevance of miR-137 and its liver microenvironment regulatory target gene AFM in hepatocellular carcinoma
Prognostic relevance of miR-137 and its liver microenvironment regulatory target gene AFM in hepatocellular carcinoma
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miR-137及其肝脏微环境调控靶基因AFM在肝细胞癌中的预后相关性
DOI：
10.1002/jcp.27855
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发表时间：
2018
影响因子：
5.6
通讯作者：
Minjie Wei
中科院分区：
文献类型：
--
作者：
Qian Wei;Lin Zhao;Longyang Jiang;Jia Bi;Zhaojin Yu;Lan Zhao;Xinyue Song;Mingli Sun;Yuzong Chen;Minjie Wei
miR-137已被确定为潜在的肝细胞癌（HCC）预后生物标志物。高度相关的HCC预后生物标志物可能来源于miR-137与其参与肝脏微环境调节的靶基因的组合。该研究旨在发现这种组合在比先前研究显著更多数量的HCC患者中比单独的miR-137或its.target基因具有改善的HCC预后性能。对癌症和非癌组织之间差异表达的微小RNA（miRNAs）的分析再次证实miR-137是最相关的预后miRNAs之一，375例HCC患者和50例正常病例的数据来自癌症基因组图谱（TCGA）数据集。通过已建立的检索方法和HCC患者的Kaplan-Meier生存分析来确定靶基因。使用HCC患者的Kaplan-Meier生存分析来评估总生存期（OS）和无复发生存期（RFS）。考克斯比例风险回归分析表明，miR-137及其靶基因AFM组合是HCC中OS和RFS的独立预后因素。体外实验证实miR-137可以与AFM的3 ′-非翻译区结合，促进肝癌细胞株的侵袭和转移。miR-137及其肝脏微环境调控靶基因AFM的表达与HCC进展显著相关，这表明它们可能成为HCC的预后生物标志物。关键词：AFM，联合生物标志物，肝细胞癌，微环境，miR-137，预后
MiR‐137 has been identified as potential hepatocellular carcinoma (HCC).prognostic biomarkers. Highly relevant HCC prognostic biomarkers may be.derived from combinations of miR‐137 with its target genes involved in the.regulation of liver microenvironment. This study aimed at the discovery of such a.combination with improved HCC prognosis performance than miR‐137 or its.target gene alone in a significantly higher number of HCC patients than previous.studies. Analysis of the differentially expressed micro RNAs (miRNAs) between.cancer and noncancer tissues reconfirmed miR‐137 to be among the most relevant.prognostic miRNAs and the data of 375 HCC patients and 50 normal cases were.from the Cancer Genome Atlas (TCGA) data sets. Target genes were identified by.the established search methods and Kaplan–Meier survival analysis of HCC.patients was used to evaluate the overall survival (OS) and recurrence‐free.survival (RFS). Cox proportional hazards regression indicated that the miR‐137.and its target gene AFM combination is an independent prognostic factor for the.OS and RFS in HCC. In vitro experiments validated that miR‐137 could bind to.3′‐untranslated region of the AFM and promote the invasion and metastasis of.HCC cell lines. The expressions of miR‐137 and its liver microenvironment.regulatory target gene AFM in combination significantly correlated with HCC.progression in a higher number of patients than in previous studies, which.suggested their potential as prognostic biomarkers for HCC..KEYWORDS.AFM, combination biomarkers, hepatocellular carcinoma, microenvironment, miR‐137,prognosis