NO- activates soluble guanylate cyclase and Kv channels to vasodilate resistance arteries
NO- activates soluble guanylate cyclase and Kv channels to vasodilate resistance arteries
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DOI：
10.1161/01.hyp.0000072010.54901.de
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发表时间：
2003-06-01
期刊：
影响因子：
8.3
通讯作者：
Kemp-Harper, BK
中科院分区：
文献类型：
--
作者：
Irvine, JC;Favaloro, JL;Kemp-Harper, BK
一氧化氮（NO）在血管张力的调节中起重要作用。传统上，其血管舒张活性归因于NO的自由基形式（NOcircle），然而NO的还原形式（NO-）也是内源性产生的，并且是大导管动脉的有效血管舒张剂。NO-在阻力血管中的作用尚不清楚。本研究检测了大鼠离体肠系膜阻力样小动脉中NO-的活性，并对其作用机制进行了表征。使用标准肌造影技术，比较了NOcircle（NO气体溶液）、NO-（Angeli盐）和NO供体硝普钠的血管舒张特性。在存在羧基-PTIO（NOcircle清除剂）的情况下，对Angeli盐的舒张反应（pEC（50）=7.51+/-0.13，R-max=95.5+/-1.5%）不变，但对NOcircle和硝普钠的舒张反应被抑制。L-半胱氨酸（NO清除剂）可降低心肌细胞对安氏盐的敏感性（P
Nitric oxide (NO) plays an important role in the control of vascular tone. Traditionally, its vasorelaxant activity has been attributed to the free radical form of NO (NOcircle), yet the reduced form of NO (NO-) is also produced endogenously and is a potent vasodilator of large conduit arteries. The effects of NO- in the resistance vasculature remain unknown. This study examines the activity of NO- in rat small isolated mesenteric resistance-like arteries and characterizes its mechanism(s) of action. With the use of standard myographic techniques, the vasorelaxant properties of NOcircle (NO gas solution), NO- (Angeli's salt), and the NO donor sodium nitroprusside were compared. Relaxation responses to Angeli's salt (pEC(50)=7.51+/-0.13, R-max=95.5+/-1.5%) were unchanged in the presence of carboxy-PTIO (NOcircle scavenger) but those to NOcircle and sodium nitroprusside were inhibited. L-Cysteine (NO- scavenger) decreased the sensitivity to Angeli's salt (P