ELECTROPHYSIOLOGICAL PROPERTIES OF MEMBRANE AND ACETYLCHOLINE-RECEPTOR IN DEVELOPING RAT AND CHICK MYOTUBES
ELECTROPHYSIOLOGICAL PROPERTIES OF MEMBRANE AND ACETYLCHOLINE-RECEPTOR IN DEVELOPING RAT AND CHICK MYOTUBES
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DOI：
10.1085/jgp.66.3.327
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发表时间：
1975-01-01
影响因子：
3.8
通讯作者：
FAMBROUGH, DM
中科院分区：
文献类型：
--
作者：
RITCHIE, AK;FAMBROUGH, DM
本文研究了大鼠和鸡肌管在不同发育阶段的膜特性。随着肌管从成肌细胞发育为大的多核纤维，大鼠和鸡的静息膜电位（Em）从-8 mV增加到-55 mV。在大鼠肌管中，这种增加并不伴随着显着的变化，在特定的膜电阻率或Na+和K+离子分布的变化。我们也没有观察到一个显着的生电成分，在正常情况下，成熟大鼠肌管的休息EM。已经观察到膜对K+相对于Na+离子的被动渗透性逐渐增加，这可以解释Em随发育的变化。相反，在膜的离子选择性的变化，我们已经发现，大鼠和鸡肌管的ACh受体的离子选择性保持不变，在同一时期的肌管发展。
Membrane properties of rat and chick myotubes in various stages of development were studied. Resting membrane potentials (Em) increased from -8 to -55 mV in both rat and chick as the myotubes developed from myoblasts to large multinucleated fibers. In the rat myotubes, this increase was not accompanied by significant changes in specific membrane resistivity or changes in Na+ and K+ ion distribution. Nor have we observed a significant electrogenic component to the resting Em of mature rat myotubues under normal circumstances. A progressive increase in the passive permeability of the membrane to K+ relative to Na+ ions has been observed which can account for the changes in Em with development. In contrast to the changes in the ionic selectivity of the membrane, we have found that the ionic selectivity of the ACh receptor of rat and chick myotubes remains constant during the same period of myotube development.