Evaluation of the Effects of Reactive Oxygen Species on Growth of Escherichia coli by Electron Spin Resonance Spin Trapping
Evaluation of the Effects of Reactive Oxygen Species on Growth of Escherichia coli by Electron Spin Resonance Spin Trapping
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DOI：
10.3136/fstr.25.443
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发表时间：
2019-05-01
影响因子：
0.6
通讯作者：
Okamoto, Susumu
中科院分区：
文献类型：
--
作者：
Kameya, Hiromi;Kanazaki, Mika;Okamoto, Susumu
活性氧（ROS）在培养基中的积累可能会抑制微生物的生长，并阻止食品中有害细菌的检测。在这项研究中，我们研究了ROS（羟基自由基和过氧化氢）和大肠杆菌生长之间的关系，使用电子自旋共振，同时检测和定量多个氧自由基。ROS对正常大肠杆菌的生长影响不大。大肠杆菌细胞。热处理的E.大肠杆菌细胞对活性氧敏感，加入硫代硫酸钠或丙酮酸钠后，菌落形成单位数增加了10倍，这两种物质都能抑制培养基中的活性氧。这两种化合物有效地清除羟基自由基，而丙酮酸钠消除过氧化氢在更大程度上比硫代硫酸钠。我们的研究结果表明，羟基自由基水平是更关键的生长和生存的损害E。coli细胞。
Accumulation of reactive oxygen species (ROS) in the culture medium may inhibit the growth of microorganisms and prevent the detection of harmful bacteria in foods. In this study, we investigated the relationship between ROS (hydroxyl radical and hydrogen peroxide) and Escherichia coli growth using electron spin resonance, which detects and quantifies multiple oxygen free radicals simultaneously. ROS had little effect on the growth of normal E. coli cells. In contrast, heat-treated E. coli cells were sensitive to ROS, and the number of colony-forming units increased 10-fold with the addition of sodium thiosulfate or sodium pyruvate, both of which scavenge ROS in the culture medium. Both compounds efficiently scavenged hydroxyl radical, while sodium pyruvate eliminated hydrogen peroxide to a greater extent than sodium thiosulfate. Our results imply that hydroxyl radical levels are more critical for the growth and survival of damaged E. coli cells.