Protective Effect of Blue-M1 against Oxidative Stress on COS-1 Cells
Protective Effect of Blue-M1 against Oxidative Stress on COS-1 Cells
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Blue-M1 对 COS-1 细胞氧化应激的保护作用
DOI：
10.1271/bbb.68.247
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发表时间：
2004
期刊：
影响因子：
--
通讯作者：
F. Hayase
中科院分区：
文献类型：
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作者：
T. Usui;Satomi Shizuuchi;Hirohito Watanabe;F. Hayase
Blue-M1是由木糖和甘氨酸在美拉德反应中形成的蓝色颜料。前期研究表明，Blue-M1在体外具有清除羟自由基、抑制亚油酸自氧化的作用。研究了Blue-M1对2，2 ′-偶氮二（2-脒基丙烷）二盐酸盐（AAPH）诱导的COS-1细胞毒性的保护作用。将COS-1细胞在含有AAPH的DMEM培养基中在37°C下培养24小时，所述DMEM培养基含有或不含有Blue-Ml。Blue-M1可降低AAPH对COS-1细胞的毒性作用，并呈剂量依赖性。此外，用二苯基-1-芘基膦（DPPP）作为用于检测脂质过氧化物的试剂处理COS-1细胞，然后在含有AAPH的DMEM培养基中在37°C下培养6小时，所述AAPH含有或不含有Blue-M1。Blue-M1可抑制AAPH诱导的COS-1细胞膜过氧化反应，且呈剂量依赖性。这些结果表明，Blue-M1可防止氧化性细胞损伤。因此，Blue-M1将是一种抗氧化剂，可以保护生命系统免受氧化应激。
Blue-M1 is a blue pigment formed from xylose and glycine in the Maillard reaction. Previous work revealed that Blue-M1 scavenged hydroxyl radicals, and prevented the autoxidation of linoleic acid in vitro. We investigated the protective effect of Blue-M1 for 2,2′-azobis(2-amidino-propane)dihydrochloride (AAPH)-induced toxicity in COS-1 cells. COS-1 cells were cultured in AAPH containing DMEM medium with or without Blue-M1 at 37°C for 24 h. Blue-M1 decreased the AAPH-induced toxicity in COS-1 cells, and this effect was dose-dependent. Furthermore, COS-1 cells were treated with diphenyl-1-pyrenylphosphine (DPPP), as a reagent for the detection of lipid peroxide, and then were cultured in AAPH containing DMEM medium with or without Blue-M1 at 37°C for 6 h. Blue-M1 prevented the AAPH-induced peroxidation of cell membrane on COS-1 cells, and this effect was also dose-dependent. These results suggest that Blue-M1 prevents the oxidative cell injury. Therefore, Blue-M1 will be an antioxidant, which protect against the oxidative stress in living systems.