Strain difference of cadmium accumulation by liver slices of inbred Wistar-Imamichi and Fischer 344 rats.
Strain difference of cadmium accumulation by liver slices of inbred Wistar-Imamichi and Fischer 344 rats.
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DOI：
10.1016/j.tiv.2007.09.013
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发表时间：
2008-03
期刊：
影响因子：
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通讯作者：
H. Shimada;A. Yasutake;Takaomi Hirashima;Yasutaka Takamure;T. Kitano;M. Waalkes;Y. Imamura
中科院分区：
文献类型：
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作者：
H. Shimada;A. Yasutake;Takaomi Hirashima;Yasutaka Takamure;T. Kitano;M. Waalkes;Y. Imamura
研究了近交系Wistar-Imamichi(WI)大鼠和Cd敏感Fischer 344(F344)大鼠肝脏切片对Cd积累的品系差异。WI大鼠肝切片对Cd的蓄积量显著低于F344大鼠，提示这两个品系大鼠肝细胞转运Cd的差异与品系有关。此外，在Wi和F344大鼠的肝脏切片中，锌的积累也有相似的菌株差异。当培养液中加入锌和镉时，F344肝切片对镉的积累量减少。此外，在F344肝切片中加入Cd后，锌的积累量显著减少。这些结果表明，肝脏对Cd的积累可能至少部分是由锌转运系统介导的。然而，我们发现在WI和F344大鼠之间，肝脏中的ZIP3和ZT3的转录水平没有品系差异。进一步的工作正在进行中，以确定导致在WI和F344大鼠中看到的肝脏镉积累的菌株差异的转运体。
Strain difference in the accumulation of cadmium (Cd) by liver slices was examined in inbred Cd-resistant Wistar-Imamichi (WI) and Cd-sensitive Fischer 344 (F344) rats. The accumulation of Cd by liver slices of WI rats was significantly lower than that of F344 rats, suggesting strain-related differences in the transport of Cd into the liver cells of these two rat strains. In addition, a similar strain difference was observed in the accumulation of zinc (Zn) by liver slices from WI and F344 rats. Cd accumulation by F344 liver slices decreased when Zn was added to the medium in combination with Cd. Furthermore, in F344 liver slices, Zn accumulation was significantly decreased when Cd was added to the medium. These results suggest that the accumulation of Cd by the liver is probably mediated, at least in part, by Zn transport systems. However, we found no strain difference in hepatic ZnT3 or ZIP3 transcript levels between WI and F344 rats. Further work is in progress to identify the transporter that causes the strain differences in hepatic Cd accumulation seen with WI and F344 rats.