Illuminating necrosis: From mechanistic exploration to preclinical application using fluorescence molecular imaging with indocyanine green.
Illuminating necrosis: From mechanistic exploration to preclinical application using fluorescence molecular imaging with indocyanine green.
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DOI：
10.1038/srep21013
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发表时间：
2016-02-11
影响因子：
4.6
通讯作者：
Tian J
中科院分区：
文献类型：
--
作者：
Fang C;Wang K;Zeng C;Chi C;Shang W;Ye J;Mao Y;Fan Y;Yang J;Xiang N;Zeng N;Zhu W;Fang C;Tian J
组织坏死通常伴随着一系列严重疾病的发展。因此，通过有效的成像技术对坏死组织进行高灵敏度的检测和精确的边界勾画是临床治疗的关键，然而，到目前为止，还没有一种成像方法取得令人满意的结果。尽管荧光分子成像(FMI)在这方面显示出潜力，但还没有开发出有效的抗坏死荧光探针用于临床应用。在这里，我们证明了吲哚青绿(ICG)可以通过与脂蛋白(LP)和磷脂的相互作用而实现对坏死组织的高亲和力。通过一系列体外研究，从细胞和分子水平探讨了其作用机制。在自行设计的成像设备的帮助下，FMI成功地在不同动物模型的不同器官中实现了坏死组织的检测和实时图像引导手术。结果表明，通过精确的边界定义，可以有效地检测出直径为0.6 mm的坏死组织。我们相信，这一新发现和相关的成像技术将在不久的将来提高个性化和精确化的外科手术。
Tissue necrosis commonly accompanies the development of a wide range of serious diseases. Therefore, highly sensitive detection and precise boundary delineation of necrotic tissue via effective imaging techniques are crucial for clinical treatments; however, no imaging modalities have achieved satisfactory results to date. Although fluorescence molecular imaging (FMI) shows potential in this regard, no effective necrosis-avid fluorescent probe has been developed for clinical applications. Here, we demonstrate that indocyanine green (ICG) can achieve high avidity of necrotic tissue owing to its interaction with lipoprotein (LP) and phospholipids. The mechanism was explored at the cellular and molecular levels through a series of in vitro studies. Detection of necrotic tissue and real-time image-guided surgery were successfully achieved in different organs of different animal models with the help of FMI using in house-designed imaging devices. The results indicated that necrotic tissue with a 0.6 mm diameter could be effectively detected with precise boundary definition. We believe that the new discovery and the associated imaging techniques will improve personalized and precise surgery in the near future.