Subgroup-E avian-leukosis-virus-associated disease in chickens.
Subgroup-E avian-leukosis-virus-associated disease in chickens.
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鸡中 E 亚组禽白血病病毒相关疾病。
DOI：
10.1101/sqb.1980.044.01.122
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发表时间：
1980
期刊：
影响因子：
--
通讯作者：
Coffin,JM
中科院分区：
文献类型：
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作者：
Robinson,HL;Pearson,MN;DeSimone,DW;Tsichlis,PN;Coffin,JM
材料和方法病毒株。RAV-1是一种外源性病毒，其env基因编码A亚群抗原。常规用于非急性ALV相关疾病实验的RAV-1分离株获自LB Crittenden（Regional Poultry Research Laboratory，Michigan）。我们在[15 B· K（-）]· K28细胞上培养了这种病毒，该细胞对所有已知的ALV亚群都是允许的（罗宾逊等人，1979）。在感染后的第三代和第五代，病毒干扰亚组-A的重叠感染，但不干扰亚组-B，-C，-D，-E，-F，或-G禽肉瘤病毒（ASV）。因此，RAV-1原种仅含有A亚群env基因，RAV-60是外源和内源病毒信息的E亚群重组体（Hanafusa et al. 1970;韦斯et al. 1973;海沃德和Hanafusa 1975; Shoyab和Baluda 1976; Rettenmeir和Hanafusa 1977）。从H.和T. Hanafusa（洛克菲勒大学，纽约）。这些病毒的名称和来源总结见表1。这些病毒由Hanafusas进行了两次终点纯化。在我们的实验室中，它们在[15 B· K（-）] x K28细胞中生长，在那里它们干扰ASV的亚群-E和仅亚群-E的超感染。
MATERIALS AND METHODSViral strains. RAV-1 is an exogenous virus with an env gene that codes for subgroup-A antigens. An isolate of RAV-1 that is routinely used for experiments on nonacute ALV-associated disease was obtained from LB Crittenden (Regional Poultry Research Laboratory, Michigan). We grew this virus on [15B• K (-)]• K28 cells, which are permissive for all known subgroups of ALVs (Robinson et al. 1979). At three passages and five passages after infection, the virus interfered with superinfection by subgroup-A but not subgroup-B,-C,-D,-E,-F, or-G avian sarcoma viruses (ASVs). Thus, the RAV-1 stock contained only subgroup-A env genes.RAV-60s are subgroup-E recombinants of exogenous and endogenous virus information (Hanafusa et al. 1970; Weiss et al. 1973; Hayward and Hanafusa 1975; Shoyab and Baluda 1976; Rettenmeir and Hanafusa 1977). Four independent isolates of RAV-60s (NY201, NY202, NY203, and NY204) were obtained from H. and T. Hanafusa (Rockefeller University, New York). The designations and origins of these viruses are summarized in Table 1. These viruses were end-point-purified two times by the Hanafusas. In our laboratory they were grown in [15B• K (-)] x K28 cells where they interfered with superinfection by subgroup-E, and only subgroup-E, pseudotypes of ASVs.