Expression of K2P5.1 potassium channels on CD4+ T lymphocytes correlates with disease activity in rheumatoid arthritis patients.
Expression of K2P5.1 potassium channels on CD4+ T lymphocytes correlates with disease activity in rheumatoid arthritis patients.
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DOI：
10.1186/ar3245
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发表时间：
2011-02-11
影响因子：
4.9
通讯作者：
Meuth SG
中科院分区：
文献类型：
--
作者：
Bittner S;Bobak N;Feuchtenberger M;Herrmann AM;Göbel K;Kinne RW;Hansen AJ;Budde T;Kleinschnitz C;Frey O;Tony HP;Wiendl H;Meuth SG
CD4+ T 细胞表达 K2P5.1（TWIK 相关酸敏感钾通道 2 (TASK2)；KCNK5），它是双孔域钾通道家族的成员，已被证明会影响 T 细胞效应器功能。最近，研究表明 K2P5.1 在（自身免疫）T 细胞刺激后上调。本研究的目的是将类风湿性关节炎 (RA) 患者 T 细胞上 K2P5.1 的表达水平与这些患者的疾病活动性相关联。通过RT-PCR测量58例RA患者外周血中K2P5.1的表达水平，并与疾病活动参数（C反应蛋白水平、红细胞沉降率、疾病活动评分（DAS28）评分）相关。对 20 名接受治疗改变的患者进行了为期六个月的随访。此外，还研究了滑液和滑液活检中表达 K2P5.1 的 T 淋巴细胞。 CD4+ T 细胞中的 K2P5.1 表达水平与 RA 患者的 DAS28 评分密切相关。血清学炎症参数（红细胞沉降率、C 反应蛋白）也存在类似的相关性。此外，滑液来源的 T 细胞的 K2P5.1 表达水平高于外周血 T 细胞。个体患者的前瞻性数据显示，在六个月的纵向随访期间，K2P5.1 表达与疾病活动参数具有平行行为。在横断面和纵向观察中，RA 患者的疾病活动度与外周血 CD4+ T 淋巴细胞中 K2P5.1 的表达水平密切相关。需要进一步的研究来调查确切的病理生理机制，并评估 K2P5.1 作为疾病活动和鉴别诊断的潜在生物标志物的可能性。
CD4+ T cells express K2P5.1 (TWIK-related acid-sensitive potassium channel 2 (TASK2); KCNK5), a member of the two-pore domain potassium channel family, which has been shown to influence T cell effector functions. Recently, it was shown that K2P5.1 is upregulated upon (autoimmune) T cell stimulation. The aim of this study was to correlate expression levels of K2P5.1 on T cells from patients with rheumatoid arthritis (RA) to disease activity in these patients. Expression levels of K2P5.1 were measured by RT-PCR in the peripheral blood of 58 patients with RA and correlated with disease activity parameters (C-reactive protein levels, erythrocyte sedimentation rates, disease activity score (DAS28) scores). Twenty patients undergoing therapy change were followed-up for six months. Additionally, synovial fluid and synovial biopsies were investigated for T lymphocytes expressing K2P5.1. K2P5.1 expression levels in CD4+ T cells show a strong correlation to DAS28 scores in RA patients. Similar correlations were found for serological inflammatory parameters (erythrocyte sedimentation rate, C-reactive protein). In addition, K2P5.1 expression levels of synovial fluid-derived T cells are higher compared to peripheral blood T cells. Prospective data in individual patients show a parallel behaviour of K2P5.1 expression to disease activity parameters during a longitudinal follow-up for six months. Disease activity in RA patients correlates strongly with K2P5.1 expression levels in CD4+ T lymphocytes in the peripheral blood in cross-sectional as well as in longitudinal observations. Further studies are needed to investigate the exact pathophysiological mechanisms and to evaluate the possible use of K2P5.1 as a potential biomarker for disease activity and differential diagnosis.
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影响因子：
27.4
作者：
Wells G;Becker JC;Teng J;Dougados M;Schiff M;Smolen J;Aletaha D;van Riel PL
通讯作者：
van Riel PL
影响因子：
4.9
作者：
Goronzy JJ;Weyand CM
通讯作者：
Weyand CM
影响因子：
27.4
作者：
Jones G;Sebba A;Gu J;Lowenstein MB;Calvo A;Gomez-Reino JJ;Siri DA;Tomsic M;Alecock E;Woodworth T;Genovese MC
通讯作者：
Genovese MC
影响因子：
4.8
作者：
Meuth, Sven G.;Bittner, Stefan;Wiendl, Heinz
通讯作者：
Wiendl, Heinz
影响因子：
2.2
作者：
Matsui, Toshihiro;Komiya, Akiko;Tohma, Shigeto
通讯作者：
Tohma, Shigeto