Analysis of compartmentation of ATP in skeletal and cardiac muscle using 31P nuclear magnetic resonance saturation transfer.
Analysis of compartmentation of ATP in skeletal and cardiac muscle using 31P nuclear magnetic resonance saturation transfer.
复制标题
使用 31P 核磁共振饱和转移分析骨骼肌和心肌中 ATP 的区室。
DOI：
10.1016/s0006-3495(87)83416-1
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发表时间：
1987
影响因子：
3.4
通讯作者：
Ingwall,JS
中科院分区：
文献类型：
--
作者：
Zahler,R;Bittl,JA;Ingwall,JS
我们已经开发了一个模型，用于分析的正向肌酸激酶反应在肌肉中测量的核磁共振（NMR）技术的磁化转移。该模型考虑到在一些NMR磁化传递数据中观察到的双指数行为，允许存在两个ATP池，一个是NMR可见的（NMR-VIS），另一个是NMR不可见的（NMR-INVIS）。我们已经将该模型应用于骨骼肌和心肌中肌酸激酶反应的实验数据，以研究肌酸激酶速率常数和流量对工作负荷的依赖性，并考虑心脏和骨骼肌之间的差异。结果表明，核磁共振不同的ATP池存在于心脏和骨骼肌，磷酸盐交换与肌酸激酶催化的这个池增加工作量。结果也同意先前发表的估计线粒体易位酶和净ATP合成率通过传统的生化方法获得。
We have developed a model for the analysis of the forward creatine kinase reaction in muscle as measured by the nuclear magnetic resonance (NMR) technique of magnetization transfer. The model, accounting for the double-exponential behavior observed in some NMR magnetization transfer data, allows for the existence of two ATP pools, one that is NMR-visible (NMR-VIS) and another that is NMR-invisible (NMR-INVIS). We have applied the model to experimental data for the forward creatine kinase reaction in skeletal and cardiac muscles to study the dependence of the creatine kinase rate constants and fluxes on workload and to account for the differences between heart and skeletal muscle. The results suggest that an NMR-distinct ATP pool exists in both heart and skeletal muscles, and that phosphate exchange with this pool catalyzed by creatine kinase increases with increased workload. The results also agree with previously published estimates of the rates of mitochondrial translocase and net ATP synthesis obtained by traditional biochemical methods.