Imaging morphogenesis, in Xenopus with Quantum Dot nanocrystals
Imaging morphogenesis, in Xenopus with Quantum Dot nanocrystals
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DOI：
10.1016/j.mod.2009.07.008
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发表时间：
2009-10-01
影响因子：
2.6
通讯作者：
Skourides, Paris A.
中科院分区：
文献类型：
--
作者：
Stylianou, Panayiota;Skourides, Paris A.
中胚层迁移是一种形态发生运动，发生在非洲爪哇原肠发育过程中。对中胚层迁移和其他形态发生运动的研究主要基于体外实验，因为无法成像不透明胚胎中的深层组织运动。我们首次报道了使用近红外量子点(NIR QD‘s)以单细胞分辨率对体内中胚层迁移进行成像，并提供关于迁移速率的体内定量数据。此外，我们使用QD‘s来研究粘着斑激酶(FAK)在这一运动中的作用。抑制FAK在体外和体内均可阻止中胚层的扩散和迁移，而不影响收敛延伸，突出了两种运动之间的分子差异。这些结果为FAK和粘着斑在原肠形成过程中的作用提供了新的见解，并为体内形态发生的研究提供了新的工具。(C)2009爱思唯尔爱尔兰有限公司。保留所有权利。
Mesoderm migration is a well studied morphogenetic movement that takes place during Xenopus gastrulation. The study of mesoderm migration and other morphogenetic movements has been primarily based on in vitro assays due to the inability to image deep tissue movements in the opaque embryo. We are the first to report the use of Near Infra Red Quantum Dots (NIR QD's) to image mesoderm migration in vivo with single cell resolution and provide quantitative in vivo data regarding migration rates. in addition we use QD's to address the function of the focal adhesion kinase (FAK) in this movement. inhibition of FAK blocks mesoderm spreading and migration both in vitro and in vivo without affecting convergent extension highlighting the molecular differences between the two movements. These results provide new insights about the role of FAK and of focal adhesions during gastrulation and provide a new tool for the study of morphogenesis in vivo. (C) 2009 Elsevier Ireland Ltd. All rights reserved.